RFLP

In this study, cry-type genes from a new Bacillus thuringiensis GGD-4 stain, isolated by dilution-plate method from Lianyungang, had been identified using PCR-RFLP identification system, and SDS-PAGE analysis and bioassay had been performed.

利用稀释平板培养法从连云港地区的土壤中分离了一株苏云金芽孢杆菌新菌株GGD-4，通过PCR-RFLP鉴定体系和SDS-PAGE方法分析了此菌株中cry基因类型和表达蛋白。

31 isolates were gotten after separating, purifying and nodulation checking and researched by methods of 16S rDNA PCR RFLP and BOX-PCR.

通过分离、纯化、回接结瘤测定共获得了31株待测菌，并采用16S rDNA PCR RFLP及BOX-PCR等方法对这些菌进行了研究。

Modified PCR-RFLP determination of relative content of heterogenous mitochondrial gene

改良PCR-RFLP法测定异质性线粒体基因的相对含量

Molecular detection and early diagnosis of Fusarium with PCR-RFLP program

枯萎病菌的PCR-RFLP分子检测和早期诊断

Clinical Significance of HBV Basal Core Promoter Double-nucleotide Mutation Detected by PCR-RFLP

PCR-RFLP分析HBV核心启动子基因突变的临床意义

16S rDNA-RFLP analysis of structure and diversity of an aerobic microbial community degrading hexachlorobenzene

16S rdna-RFLP分析六氯苯好氧降解菌群的结构及其多样性

Sequencing and PCR-RFLP analysis of the partial 5 ′ flanking non-translated region of yak α-lactalbumin gene

牦牛α-乳清蛋白基因5′-非翻译区部分序列测定及PCR-RFLP分析

PCR-RFLP Analysis on Edaphon Community Structure in Eucalyptus Plantation

PCR-RFLP分析桉树人工林土壤微生物的群落结构

Molecular differentiation of Salmonella pullorum from Salmonella gallinarum by PCR-RFLP

鸡白痢和鸡伤寒沙门氏菌的PCR-RFLP分子鉴别

Popular genetic markers in the aquaculture community include mitochondrial DNA, RFLP, RAPD, AFLP, microsatellite, SNP, and EST markers.

目前,在水产养殖种类中使用的遗传标记主要包括线粒体DNA 、 RFLP 、 RAPD 、 AFLP 、 微卫星 、 SNP和EST标记.

PCR-RFLP method was adopted to examine the genetic variety of genes ESR and FSH β in 5 pig populations ( Landrace and Large White from the farm of Zhenghong; Landrace, Large White, Duroc from the farm in the agriculture institute of Yiyang).

本研究采用PCR-RFLP方法检测了5个猪群体（正虹种猪场长白、大白；益阳农科所种猪场杜洛克、长白、大白）ESR和FSHP基因的遗传变异。

Methods HPV DNA was detected using polymerase chain reaction ( PCR) with a consensus primer and typed by using restriction fragment length polymorphism ( RFLP) method in 151 paraffin-embedded biopsy specimens from seven kinds of external genital epidermal proliferative lesions.

方法采用通用型引物PCR方法对151例外生殖器表皮增殖性病变进行HPV DNA的检测，应用限制性内切酶片段长度多态性（RFLP）方法进行HPV DNA分型。

Molecular Identification of Fallopia multiflora by PCR-RFLP Based on rDNA ITS Sequence

基于rDNA ITS序列的何首乌PCR-RFLP分子鉴别

Methods This study covered 478 cases with type 2 diabetes mellitus without consanguinity and 430 subjects with normal glucose tolerance without DM family history. The mutations were determined by PCR-RFLP.

方法对无血缘关系的478例2型糖尿病患者进行研究，同时选择430例无糖尿病家族史的糖耐量正常者作为对照，用PCR RFLP筛选点突变。

These ESTs were assigned to chromosomes based on RFLP mapping and rice genome database search.

我们通过基因组数据库同源查找和RFLP定位将这些EST整合到连锁图谱上。

The genetic map with 221 markers was constructed by Crop Genetic Improvement National Key Laboratory in Huazhong Agricultural University. The markers included 175 RFLP markers, 45 SSR markers and 1 morphological marker.

所用的遗传图谱是由华中农大作物改良国家重点实验室构建的，共有221个标记，其中包括175个RFLP标记、45个SSR标记和1个形态标记。

Application of semi-quantitative polymerase chain reaction in the PCR-RFLP analysis to detect gene polymorphism

半定量聚合酶链反应在PCR-RFLP酶切法测定基因多态性中的应用

And the results obtained in this study might be referential AFLP and RFLP analysis.

这一结论受研究对象的影响小,在其它类似的研究中或许具有一定的参考价值.

The restriction fragment length polymorphism ( RFLP) of mitochondrial DNA ( mtDNA) of cobia was studied using 19 restriction endonucleases recognizing 5, 6 base pairs. The physical map of mtDNA has been constructed based on 6 endonucleases selected from 20 restriction endonucleases.

用19种识别5、6碱基的限制性内切酶对军曹鱼的mtDNA RFLP进行了分析研究，构建其mtDNA物理图谱；

RFLP ( restriction fragment length polymorphism) analysis showed that the transposon is 3 cM ( centiMorgan) away from the S locus and retains high transpositional activity with a germinal excision frequency of 20%.

限制性酶切片段长度多态性（RFLP）的分析证明该转座子距S位点约3cM（centiMorgan），并且保持了较高的转位活性，其配子切除频率高达20%。

【 Method 】 Twenty isolates from Glycyrrhiza spp. were characterized by numerical taxonomy, 16S rDNA PCR-RFLP analysis and BOX-PCR fingerprinting to describe the phenotypic and genetic diversity.

【方法】采用表型数值分类、16s rDNA PCR-RFLP分析和BOX-PCR指纹图谱分析的方法对北方地区的甘草根瘤菌进行表型、遗传多样性分析。

Detection of an inherited defect: von Willebrand Disease by PCR-RFLP in five police dog breeds

利用PCR-RFLP检测五大不同品种警犬遗传缺陷&血管性假性血友病

Restriction fragment length polymorphism ( RFLP ) technique used in cancer research.

1983年限制片段长度多态 ( RFLP ) 技术在癌症研究中应用.

Objective To establish the specific 16S 23S rRNA gene spacer regions map of different bacteria by PCR, RFLP ( restriction fragment length polymorphism), DNA clone and sequences analysis.

目的建立检测不同菌种细菌的16S23S RRNA基因区间的特异图谱。

Detecting HPV DNA in Tissues of External Genital Squamous Cell Carcinoma in Situ by PCR-RFLP Technique

PCR-RFLP法检测外生殖器表皮原位癌组织HPV DNA

Conclusion RFLP of ER gene associated with BMD in postmenopausal healthy women and this might explain the cause of postmenopausal osteoporosis in Chinese women.

结论ER基因RFLP与绝经后妇女BMD有关，此即为中国妇女绝经后骨质疏松症发病的原因。

RFLP linkage analysis of X-linked muscular dystrophy

X&连锁肌营养不良症的RFLP连锁分析

Polymorphism Analysis of Goat Growth Hormone ( GH) Gene by PCR-RFLP

山羊生长激素基因PCR-RFLP多态性分析

Compared with RFLP, RAPD, the great number of fragment can be visualized simultaneously by AFLP.

与RFLP 、 RAPD相比, AFLP具有在一次试验中可同时观察到大量的限制性片段的优点.

PCR/ RFLP technique was used to detect spotted fever group rickettsiae ( SFGR) in ticks and small mammals collected in eleven scenic spots of Beijing suburb. We not only detected Rickettsia sibirica in D.

用PCR／RFLP技术对北京郊区11个旅游景点的媒介蜱及小哺乳动物进行斑点热群立克次体的检测，并进行了病原体分离和鉴定。