denaturing

Use alkaline lysis method to extract the plasmid DNA. DNA constructs were identified by Pst I/ Bgl II digestion, denaturing polyacrylamide gel electrophoresis and DNA sequence analysis.

随机挑选单菌落小规模培养，碱裂解法提取质粒DNA，PstⅠ／BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析，筛选含有不同等位基因的重组质粒后测序。

The polypeptide of interest was purified by Ni2+ affinity chromatography under denaturing conditions, followed by renaturation of the recombinant protein by dialysis, and digestion with enterokinase to release the chimeric peptide.

经表达条件优化，大量制备重组蛋白，变性条件下进行镍离子亲和层析纯化。对纯化后的重组蛋白进行复性，用肠激酶切割复性蛋白以获得目的多肽。

Low rotary speed during grinding prevents protein denaturing under high temperature caused by grinding.

研磨转速低,能有效避免蛋白质因研磨产生高温而变性.

The 16S rDNA V6 region of soil and phyllosphere bacteria were amplified, and the result of DGGE ( Denaturing Gradient Gel Electrophoresis) showed ecological diversity.

利用PCR／DGGE技术，对土壤和叶面细菌总DNA16S rDNA V6可变区进行扩增，然后进行变性梯度凝胶电泳（DGGE），结果表明土壤、叶面的微生物群落表现出一定的多态性。

In this paper, several molecular methods such as fluorescence in situ hybridization ( FISH) and denaturing gradual gel electrophoresis ( DGGE) etc.

为此，在介绍变性梯度凝胶电泳（DGGE）以及荧光原位杂交（FISH）等分子生物学新技术的基础上，对这些技术在土壤微生态研究中存在的问题和发展前景做了展望；

The amplified DNA fragments are separated by denaturing acrylamide gels and detected by autoradiography.

扩增产物用变性的聚丙烯酰胺凝胶分离,然后用放射自显影检测.

Method: The genetic polymorphism of 144 unrelated individuals of Hui population and 104 individuals of Han population from Ningxia was analyzed by polymerase chain reaction ( PCR) amplification combined with denaturing polyacrylamide gel electrophoresis ( PAGE) and silver staining.

方法：采用PCR扩增技术和聚丙烯酰胺凝胶电泳方法，分析144名无血缘关系的宁夏回族和104名宁夏汉族健康个体的遗传多态性。

We applied PCR-single strand conformation polymorphism ( SSCP) technique to Ex-on 7 of PAN gene from PKU patients on non-denaturing micro-polyacrylamide gel.

应用非放射性的微型非变性聚丙烯凝胶电泳，对苯丙酮尿症病人的PAH基因Ex-on7的PCR扩增产物进行了单链构型多态性分析。

Denaturing high performance liquid chromatography ( DHPLC) is a novel high-throughput technique for screening DNA variations. It can be highly sensitive to detect mutations, SNPs, LOH and make genotype analysis.

变性高效液相色谱分析（DHPLC）是近年来发展起来的高通量筛选DNA结构变异的一项新技术，可以高灵敏地检测突变、SNP、LOH，以及进行基因型分析等。

Objective To establish and evaluate denaturing high-performance liquid chromatography ( DHPLC) as a rapid and efficient technique of detecting mutations of mitochondrion gene.

目的以变性高效液相色谱（DHPLC）技术分析检测线粒体肌病患者线粒体基因突变，以明确诊断。

Detection of the Lactic Acid Bacteria in Commercial Yoghurts by PCR-denaturing Gradient Gel Electrophoresis Determination of hydroxyproline in dairy products using amino acid analyzer

利用PCR-DGGE技术分析乳制品中的乳酸菌（英文）氨基酸自动分析仪检测乳及乳制品中羟脯氨酸

The Application of Denaturing High Performance Liquid Chromatography for Detection of Point Mutation of Familial Alzheimer Disease

变性高效液相色谱法在家族性阿尔茨海默病突变位点检测中的应用

Four microsatellite markers linked to FecB locus on 6 chromosome in ovine were analyzed for 108 animals by PCR and denaturing polyacrylamide gel electrophoresis.

选择4个位于绵羊6号染色体上且与FecB基因紧密连锁的微卫星标记，对小尾寒羊基因组进行PCR扩增后，采用最小二乘法估计各等位基因片段对产羔数的影响。

Value of human cytomegalovirus IgG avidity index measured with two protein denaturing agents for the diagnosis of primary infection during pregnancy Significance and Detection of Rubella Virus Specific IgG Avidity

两种蛋白变性剂检测巨细胞病毒IgG抗体亲和指数对孕期原发感染诊断的探讨风疹病毒特异性IgG抗体亲和力测定的临床意义

The disaccharide trehalose under stress conditions and the disaccharide could protect the biological moleculars from denaturing.

综述了胁迫条件下海藻糖的代谢调控及保护作用的研究近况.

And later in biology class, I learned that it's due to a process called denaturing, which is where the proteins will change shape and lose their ability to chemically function.

后来在生物课上，我学到这是由于一种叫变性的过程，就是蛋白质会改变形态，失去它们化学性功能。

Simultaneous detection of polymorphisms in the 3 '-and 5'-UTR of thymidylate synthase gene using denaturing high-performance liquid chromatography

应用变性高效液相色谱技术建立胸苷酸合成酶基因多态检测平台

The denaturing shall be carried out in the inedible products area of the establishment.

变性将在工厂的不适于食用的产品区完成。

The exon 28 of authenticity vWF gene was studied by PCR, denaturing gradient gel electrophoresis ( DGGE) and sequencing in the type 2A vWD.

对2A型血管性血友病的患者用多聚酶链反应、变性梯度凝胶电泳，结合测序的方法进行了基因突变的研究。

review on denaturing gradient gel electrophoresis and its application in food microbiology

变性梯度凝胶电泳技术及其在食品微生物研究中的应用

At present , denaturing-performance liquid chromatography ( DHPLC ) is the best technique platform for detecting mutation.

变性高效液相色谱 ( DHPLC ) 是目前检测基因突变的最佳技术平台之一.