dmem

Conclusion The slices from 2 or 4 week-old rat hippocampi and DMEM/ F12 medium may be the preferred choice for tau associated researches. An ideal Alzheimer's disease model may be established based on the results of these researches.

结论选取2或4周龄大鼠，应用培养基DMEM/F12更适合于脑片水平tau蛋白的相关研究，在此基础上可望建立阿尔茨海默病理想研究模型。

The proliferation and differentiation features of NSCs were observed under phase contrast microscope after the GM1 with different concentrations were added into NSCs' and serum-free DMEM/ F12 culture media.

2在NSCs培养基和DMEM/F12培养基中加入不同浓度的GM1，观察GM1对NSCs增殖和分化的影响。

The cells in the control group were added with DMEM/ F12 nutrient fluid of EGF, bFGF and B27 to culture in 5% CO2 incubator for one week at 37 ℃.

对照组细胞中直接加含表皮生长因子、碱性成纤维细胞生长因子、B27的DMEM/F12培养液，37℃，体积分数为0.05的CO2培养箱培养1周。

Umbilical artery rings and umbilical vein rings embedded in three-dimensional collagen gels in DMEM/ F12 ( 1:1) serum-free medium grew well. The new outgrows were positive by CD34 immunohistochemistry.

脐动脉环和脐静脉环在胶原凝胶和DMEM/F12（1：1）无血清培养基中生长良好，CD34免疫组织化学鉴定其为新生的血管。

Control group I ( SC medium+ NSC+ DMEM/ F12);

试验对照组1（SC培养基+NSC+DMEM/F12）；

Control group: ( DMEM/ F12+ 10% FBS), test group: brain tissue extracts.

分为对照组（DMEM/F12+10%FBS）和脑组织匀浆上清组。

Control group III ( NSC+ DMEM/ F12).

试验对照组3（NSC+DMEM/F12）。

Scaffold materials were cut into blocks with the size of 6 mm × 6 mm × 4 mm, sterilized by 60Co irradiance and infiltrated with DMEM-LG before using.

支架材料按实验需求切割成6mm×6mm×4mm大小，60Co辐照灭菌，使用前DMEM-LG浸润。

Methods The endometrial stromal cells were cultured in DMEM/ F12 medium containing different concentrations of IL-6 or IL-8 for 24 hours, then the media were collected to measure the levels of MMP-9 by ELISA.

方法分别用含不同浓度的IL-6和IL-8以及无干扰因素的培养液培养各组人子宫内膜基质细胞24h后，ELISA方法测定培养液中MMP-9的含量。

Results There were 50% survival rate of the hepatocytes in the 10th day cultured in high density containing 20% inactivated allograph male rat serum, 10U/ ml insulin culture medium, high sugar DMEM and they still remained 20% survival rate in the 14th day.

结果含20%同种异体雄性小白鼠灭活血清、10U/ml胰岛素的高糖型DMEM培养基作高密度培养时，50%的细胞成活率在第10天，第14天时仍有20%的存活率；

There are no remarkable differences in the culture of the mouse ES between GMEM and DMEM.

结果如下:GMEM与DMEM这 2种基本培养基在对ES细胞维持培养上差异不显著.

Objective: To observe the proliferation of RH strain tachyzoites of Toxoplasma gondii in media MEM and DMEM and in different host cell lines, and to compare the difference in expression of mitogen activated protein kinases ( MAPK).

目的：观察RH株弓形虫速殖子在不同培养基中及在NIH3T3和MRC5细胞内的增殖，比较其丝裂原激活蛋白激酶（MAPK）表达的差异。

Method To take sciatic nerves in 3-4 days SD rats and purify Schwann cells. A group: DMEM/ F12 contained 10% calf blood serum. B group: conditioned medium of macrophages activated by self-neural homogenate.

方法取3-4dSD乳鼠坐骨神经，纯化培养许旺细胞，A组加入含10%胎牛血清的DMEM/F12培养基，B组加入自体神经匀浆激活的巨噬细胞条件培养基；

When corpus striatum extract+ DMEM/ F12 ( 1:1) used as induction media ( corpus striatum group), 14.49% diencephalons NSCs was differentiated into dopaminergic neuron, significantly lower than inducer group.

用纹状体提取液与DMEM/F12按1∶1的比例组成的诱导液将中脑来源的14.49%的神经干细胞诱导为多巴胺能神经元，低于诱导剂组诱导分化的多巴胺能神经元。

Rat fetal neural stem cells ( rFNSCs) was separated from embryo about 14.5~ 16.5 days, and cultured in DMEM/ F12 media with additives and epidermal growth factor ( EGF) and basic fibroblast growth factor ( bFGF).

从14.5～16.5d的大鼠胚胎分离神经干细胞，培养于添加相应成分以及表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）的DMEM/F12培养液中。

Cell morphology had no obvious change in DMEM + EGF, K-SFM + BPE, and DMEM + FBS groups during the whole culture process.

DMEM+EGF 、 K-SFM+BPE 、 DMEM+FBS 组细胞均呈阴性表达.

At the third-generation ( F3) cells continued to be cultured ( 10% FBS, DMEM/ F12), and the cell population is divided into intervention group and control group, the experimental group were added to different concentrations of bFGF.

取第3代（F3）细胞继续进行培养（10%FBS，DMEM/F12），并将该细胞群分为干预组和空白对照组，实验组分别加入不同浓度梯度的bFGF。

The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture.

在DMEM培养基中进行了原代及传代培养,对其中的成纤维细胞进行分离纯化.

Methods The tissues of nucleus of inferior colliculus in newborn mice were isolated, collected and cultured with condition serum-free medium ( DMEM/ F12, B27, EGF 20 ng/ ml and bFGF 20 ng/ ml) in vitro.

方法分离、收集新生昆明小鼠下丘核区脑组织，体外经EGF和bFGF刺激下培养；

The length of process in the fetal calf serum+ DMEM/ F12 group was longer significantly than that in the newly calf born serum+ fetal calf serum+ DMEM/ F12 group in 4 days ( P < 0.01);

在4d内胎牛血清+DMEM/F12组细胞突起长度明显优于新生牛血清+胎牛血清+DMEM/F12组（P<0.01）；

After digestion, cells were cultured in DMEM/ F12 supplemented with 20% fetal calf serum.

将消化后得到的细胞用含20%胎牛血清的DMEM/F12培养基进行培养。

Group B: fibroblasts+ Schwann cells+ DMEM/ F12;

B组：成纤维细胞+雪旺细胞+DMEM/F12；

Methods Nucleus pulposus tissue taken from a one-month-old rabbit was treated by Trypsin and collagenase, and then the cells were cultured in DMEM/ F12 medium.

方法取一月龄新西兰兔的髓核，胰酶和胶原酶消化，DMEM/F12培养基中培养，倒置显微镜观察细胞形态。

The celt survival rate was higher in cryoprotectant containing K-SFM than DMEM.

以K-SFM培养液配制的冻存液冻存的细胞复苏后的存活率明显高于以DMEM配制 的培养液.

The results showed that DMEM/ F12 was a good cell culture medium for the epidermal stem cells and the epidermal stem cells can survive to the11 subculture in vitro.

结果表明，DMEM／F12是一种适合表皮干细胞体外增殖培养的培养基，在此培养体系中细胞可传代至11代。

Basal medium was composed of penicillinum, phytomycin, amphotericin B and DMEM/ F12 containing B27 annex solution of 0.02 volume fraction.

基础培养基组成：含青霉素、链霉素、两性霉素B及体积分数为0.02B27添加液的DMEM/F12。

Conclusion: Human umbilical vessel ring culture model of three dimensions in DMEM/ F12 ( 1:1) serum-free medium has been established.

结论：用无血清培养基和Ⅰ型胶原可以立体培养脐血管环。

The growth of human HF cultured in vitro varied with the different cultural media, those in DMEM supplemented with 10% calf serum tended to transform into catagen earlier.

人发游离毛囊体外培养中，培养基构成对毛囊的生长有直接影响。含小牛血清的DMEM培养基中生长的毛囊易于进入退化期；

NSC isolated from two months old rat ′ s brain region like hippocampus and striatum was cultivated in a DMEM/ F12 medium containing EGF and bFGF, and was identified with morphological character and nestin immunocytochemistry test.

将从2个月龄大鼠脑海马、纹状体等区域分离的细胞培养于含EGF和bFGF的DMEM/F12培养液中，在光镜下观察细胞的形态特征，并行神经巢蛋白（nestin）细胞化学染色。

The collected fat mesenchymal stem cells and cell nucleus pulposus with DMEM/ F12 ( 1: 1) medium monolayer culture respectively. 2.

所收集的脂肪间充质干细胞和髓核细胞用DMEM/F12（1：1）培养液分别单层培养。