High Expression of CSFV E0 Protein and Establishment of an Indirect ELISA for Detection of Antibodies

Cloning of E_2 gene of CSFV and construction of Pichia pastoris transfer vector

猪瘟病毒分离株E2基因的克隆与酵母表达系统转移载体的构建

Construction and Expression of the E2 of CSFV and Swine IgG Heavy Chain Fused Gene

猪瘟病毒保护抗原E2和猪IgG重链基因的合成及其表达蛋白

Preparation and Epitope Analysis of Monoclonal Antibodies against CSFV Envelope Glycoprotein E2

猪瘟病毒保护性抗原E2蛋白单抗的制备及其抗原表位的初步分析

A molecular biology method to differentiate C-strain from other strains of CSFV

一种鉴别猪瘟C-株和其他毒株的分子生物学方法

Vaccination and detection of CSFV antibody in pigs is very important.

接种疫苗并检测CSFV免疫抗体水平是预防与控制猪瘟的重要技术手段。

Establishment of RT-PCR Assay on CSFV E2 Gene and Study on CSFV Molecular Epidemiology

猪瘟病毒E2基因RT-PCR检测方法的建立及病毒分子流行病学研究

The Applied Research on the RT-PCR Nucleic Acid Detection and ELISA Antigen Detection Kit of CSFV

猪瘟病毒RT-PCR核酸检测与ELISA抗原检测试验应用研究

The results showed that the assay has high sensitivity to CSFV positive serum comparing with negative serum.

实验证明，该方法对标准的CSF阳性及阴性血清的具有良好的敏感性。

Classical swine fever ( CSFV) causes significant losses in pig industry in many countries.

猪瘟（CSFV）给养猪业都造成了巨大损失。

Preparation and Research the Biology of Monoclonal Antibodies Against the Truncated E2 Protein of CSFV

猪瘟病毒E2蛋白单克隆抗体的制备及其生物学特性的研究

The results indicated that infectious RNA of CSFV could be obtained from constructed full-length cDNA.

结果表明，所构建的全长cDNA具有感染性。

The study established a foundation for reverse genetic system of CSFV.

为猪瘟病毒C株反向遗传操作系统的建立奠定了基础。

Construction of eukaryotic expression vector of CSFV E2 genes and its secretory expression

猪瘟病毒囊膜蛋白E2基因真核分泌型表达载体的构建及其表达

Identification of a Novel Epitope on CSFV E2 Protein by Using Phage Peptide Libraries

利用随机肽库鉴定猪瘟病毒E2蛋白抗原表位

The C strain of CSFV is one of the best attenuated vaccine in the world.

猪瘟病毒（CSFV）C-株是世界范围内公认的免疫性能良好的弱毒疫苗株。

This result may have something to do with the appearance of the new variation virus of CSFV and other factors.

究其原因，可能与CSFV出现了新的变异株等因素有关。

However, the differences in CSFV antibody value among the 4 genotypes were not significant.

显著性检验结果表明，四种带型的猪其血清中猪瘟抗体效价差异不显著；

Analysis of the genome structure of CSFV by bioinformatics

用生物信息学手段辅助分析猪瘟病毒的基因结构

Research and Development Trend for the Detection of CSFV Antibody

猪瘟抗体检测技术的研究现状及发展趋势

Retroviral Vector Mediated CSFV E2 Envelope Protein Gene Expression in Eukaryotic Cells and Animal Immune Experiment

逆转录病毒载体介导的猪瘟病毒E2基因在真核细胞中表达及动物免疫试验初报