Pathological tissues of artificial infection and suspicious clinical samples were diagnosed by virus isolation and semi-nested RT-PCR. The coincidence rate of the two methods was 100%.

Using the Sen Z. Conclusions: The half nested_PCR method for detection of SENV_H was successfully established.

结论：建立的半套式PCR检测方法适用于SEN病毒H亚型检测；

Studies On Detection of Canine Distemper Virus by Semi-nested PCR

用半套式PCR检测犬瘟热病毒的研究

The products by half nested PCR using signal peptide sequences as primers were superior in quality and quantity to those by PCR with conserved sequences in the 5 ′ end variable regions as primers.

以免疫球蛋白信号肽序列为引物进行半套式PCR所得到的产物在质和量上都优越于以可变区5′末端保守序列为引物进行PCR所得到的产物。

Methods We detected the TTV DNA with chronic hepatitis B patients, chronic hepatitis C patients, hemodialysis patients and healthy blood donors by making use of semi nested PCR with TTV specific primers.

方法利用半套式PCR方法对慢性乙肝患者、慢性丙肝患者、血液透析者、健康人群中TTV的感染状况进行了筛选。

Amplification of human antibody genes with half nested PCR to increase the diversity of antibody library

半套式PCR扩增人抗体基因以增加抗体库的多样性

A Clostridium perfringens strain was isolated through pathogen isolation, morphologic observation, biochemical reaction and half-nested PCR reaction.

通过病原菌的分离培养、形态染色观察、生化鉴定和半套式PCR反应，分离出一株魏氏梭菌。

To study the correlation between bcl 2/ JH fusion gene and the occurrence of nasopharyngeal carcinoma ( NPC), bcl 2/ JH fusion gene in 41 cases of paraffin embedded NPC was investigated using semi nested in situ PCR technique.

为研究bcl-2/JH融合基因与鼻咽癌发生的关系，应用半套式原位PCR技术对41例石蜡包埋鼻咽癌组织中bcl-2/JH融合基因进行检测。

Based on the published sequences of CSFV, 26 nested and half nested PCR primers covering the genome sequence of C strain were designed.

根据已发表的猪瘟病毒（CSFV）序列设计并合成了26条覆盖全长基因组的套式和半套式PCR引物。

A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee ( Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.

基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因，建立该病毒的半套式RT-PCR检测方法。

Based on published sequences of strain C of CSFV, a specific PCR primer pair P1 N1 and a half nested PCR reverse primer N1 ′ were designed.

据已发表的CSFV C-株序列，设计合成了5′-UTR特异性PCR引物对P1-N1及半套式下游引物N1′。

Detection of Hantavirus by reverse transcriptase semi nested polymerase chain reaction and its preliminary application

逆转录-半套式聚合酶链反应检测汉坦病毒RNA及其临床初步应用

Objective To establish a new method of multiplex semi nested polymerase chain reaction ( PCR) to detect pathogens in cerebrospinal fluid ( CSF).

目的建立多重半套式聚合酶链反应（PCR）快速检测脑脊液标本中常见的病原菌。

Amplification of genes of human antibodies against HFRS virus using semi nested polymerase chain reaction and cloning, sequencing of Fd fragments

半套式PCR扩增人抗HFRS病毒抗体基因及Fd段基因的克隆和序列分析

Two serial specific primers ( P1/ P2, P2/ P3) were used in semi nested PCR, to amplify predicated gene, which is more sensitive and specific than conventional PCR.

半套式PCR是采用一对半特异性引物对目的基因进行扩增的一种方法，该方法较常规PCR的更敏感和更特异。

Methods Three specific primers to Mycobacterium tuberculosis were designed, Mycobacterium tuberculosis. Other Mycobacterium and GC-rich bacteria were analyzed by single-tube heminested PCR-SSCP technique.

方法设计三条针对结核分支杆菌的特异性引物，采用单管半套式PCR-单链构象多态性（SSCP）方法对结核分支杆菌、其它分支杆菌和富含GC碱基的细菌进行分析。

Methods: TTV DNA was determined by semi nested PCR.

方法：利用半套式PCR方法检测TTV-DNA。

Development and application of a semi-nested PCR assay for detection of porcine Actinobacillus pleuropneumoniae

猪胸膜肺炎放线杆菌半套式PCR检测方法的建立及应用

Detection of Coxiella burnetii by semi-nested PCR and DNA probe

半套式PCR和DNA探针技术检测Q热立克次体

Hybridization results illustrated that the HEV subgenomic RNAs form a 3 ′ nested set which is situated at the 3 ′ end of genome, but their 5 ′ ends do not share a common leader sequence.

通过杂交实验，发现HEV的亚基因组RNA具有典型的共3′端的半套式结构，且基因组RNA与亚基因组RNA的5′端不存在共同的引导序列。

Establishment of Half-nested PCR Method on Eperythrozoon Diagnosis

半套式PCR方法检测猪附红细胞体病的建立