western blot experiments indicated that the partially purified antibody preparations reacted specifically with the pks proteins expressed in e. coli and, as expected, also with the very large pks proteins naturally present in s. sp. fr-008.

plasmid of fowlpox recombinant virus and fowlpox virus were co-transfected into chicken embryo fibroblast ( cef) and identified by brdu drug, rt-pcr and western blotting respectively. the result showed that the e. tenella fowlpox recombinant virus ( rfpv-rhomboid) was obtained.

采用脂质体转染技术，将该质粒转染FPV282E4株感染的鸡胚成纤维细胞（CEF）中，通过BrdU药物加压筛选，并通过RT-PCR和蛋白质印迹等方法检测，筛选出一株球虫鸡痘重组病毒rFPV-Rhomboid。

methods: the expression of p-glycoprotein ( p-gp) and multidrug resistant associated protein ( mrp) of ej/ mrp, a multidrug resistant cell line of bladder carcinoma, was tested by western blot, immunocytochemistry and fcm method.

方法：利用蛋白质印迹、免疫细胞化学和FCM检测并比较膀胱癌多药耐受细胞EJ/MRP的多药耐受糖蛋白（P-gp）和多药耐受相关蛋白（MRP）的表达；

semi-quantitative rt-pcr and western blotting from gonads during various stages of sex reversal reveal that expression levels of ecam in gonads increase gradually during the transformation from female to male.

性反转不同时期性腺的半定量RT-PCR及蛋白质印迹表明，性逆转过程中性腺里ECaM的表达量逐渐增加。

results: ej/ mrp over-expressed mrp but without obvious p-gp expression. the results of protein detection by fcm were consistent with those by western blot and immunocytochemistry, but more convenient.

结果：EJ/MRP高表达MRP，无明显P-gp表达，FCM的检测结果与蛋白质印迹、免疫细胞化学的结果一致，但检测更简便；

secondly, the effects of weak light to the sugar content and sucrose synthase ( ss) regulation in source leaves and fruits of nectarine trees were studied via immunogold electron-microscopy localization and western blotting.

同时，利用胶体金免疫定位技术和蛋白质印迹技术，分别研究了弱光对源叶和果实糖含量的影响和对蔗糖合酶（SS）的调控。

rt pcr assay was used to examine tgf β 1 and ctgf mrna expression induced by ages. tgf β 1 and ctgf protein and ecm component synthesis were detected by western blot and immunohistochemistry respectively.

尾静脉注射AGE-RSP6周后，采用RT-PCR、免疫组织化学和蛋白质印迹杂交等方法检测AGEs对大鼠肾皮质TGF-β1和CTGF的mRNA和蛋白质表达及ECM蛋白成分表达的影响。

the expressions of vascular endothelial growth factor mrna and protein were detected with semi-quantitative reverse transcription-polymerase chain reaction ( rt-pcr) and western blot at 2, 8, 16 and 32 hours after culture under hypoxia and normal oxygen respectively.

在低氧和常氧培养后2,8,16,32h应用半定量反转录聚合酶链反应和蛋白质印迹技术检测其血管内皮生长因子基因mRNA和蛋白表达。

furthermore, western blot and gel electrophoresis mobility shift assay ( emsa) were performed to detect the mechanism of plc γ 1 in colorectal cancer cell adhesion to matrix.

PLCγ1在结直肠癌细胞中作用的信号转导机制采用蛋白质印迹及凝胶迁移率变动分析（EMSA）。

⒊ spc-a-1 cell total protein pretreated with therapy groups was extracted after twenty-four hours, and their bfgf and mmp-2 level was measured by western blot method.

提取各实验组作用24小时后的SPC-A-1细胞总蛋白，通过蛋白质印迹法检测bFGF和MMP-2的水平。

transcriptional and dna binding activity were analyzed by reporter gene assay and electrophoretic mobility shift assay ( emsa) respectively. western blot was used to determine the protein expression;

运用报告基因法和凝胶电泳迁移率法（EMSA）分析AP1和NFκB反式激活活性和DNA结合活性，蛋白质印迹检测蛋白质表达；

methods western blot was used to detect the vegf and er β protein expression in 86 fresh samples of human breast cancer. er α was analyzed by immunohistochemistry routinely.

方法收集乳腺癌患者手术标本86例，用蛋白质印迹法检测ERβ和VEGF含量，应用免疫组织化学的方法检测ERα的含量，对ER亚型和VEGF表达关系进行比较。

the stable transfection and expression level of ang in te-1 were determined by semi-quantitative rt-pcr and western blot.

半定量RT-PCR法及蛋白质印迹法分别观察TE-1细胞中ANG在核酸和蛋白水平表达量改变。

by western blotting, the expression of ha protein was detected in 293t cell lysate transfected by ha-vn.

蛋白质印迹法分析结果表明，HA-VN.tPA转染293T细胞后，细胞裂解液中检测出HA蛋白的表达。

finally, exploratory research was carried out in preparation of protein molecular imprinted colloid array ( mica).

最后，对蛋白质印迹光子晶体传感器（MICA）的制备技术进行了探索性的研究。

rt-pcr and western blot were taken to study the p53 expression pattern.

RT-PCR、蛋白质印迹检测p53基因的表达；

in pcos patients alone, the levels of serum fasting insulin were detected by radioimmunoassay, serum fasting glucose by glucose oxidase method.

对20例PCOS患者，用放射免疫法检测空腹胰岛素（FIN）浓度，用葡萄糖氧化酶法检测空腹血糖（FPG）浓度。

in this paper, god was immobilized in the course of gelation process of the sol of teos, and immobilized god columns were easily prepared with different carriers using this system.

本文利用硅酸乙酯（TEOS）的溶胶凝胶过程对葡萄糖氧化酶（GOD）进行了固定，并制备了不同载体下的GOD酶柱。

naphthol green b was used, for the first time, as a new mediator in an amperometric glucose biosensor. it has been shown to be a good mediator, promoting electron transfer from glucose oxidase to graphite electrode.

首次采用了染料萘酚绿B作为葡萄糖氧化酶电极的电子媒介体制备电流型葡萄糖生物传感器。

by electrochemical polymerization of pyrrole in phosphate buffer, glucose oxidase ( god) was entrapped in polypyrrole matrix ( ppy) to construct the enzyme electrode.

利用磷酸盐缓冲溶液中吡咯的电聚合，将葡萄糖氧化酶（GOD）包埋在聚吡咯（PPy）基质中以构成生物功能电极。

glucose oxidase modified electrode fabricated by layer-by-layer molecular deposition

LBL分子沉积法制备葡萄糖氧化酶电极