Reverse transcriptase PCR的例句大全

Objective To establish polymerase chain reaction ( PCR) and reverse transcriptase PCR ( RT-PCR) for detecting human cytomegalovirus ( HCMV) and to find out the relationship between diabetes mellitus ( DM) and HCMV infection.

目的建立检测人巨细胞病毒（HCMV）感染的聚合酶链反应/逆转录&聚合酶链反应（PCR/RT-PCR）方法，并探讨糖尿病与HCMV感染的关系。

Roles of the EnvZ/ OmpR two-component system in response to various stresses were investigated with mutational analysis, quantitative reverse transcriptase PCR ( qRT-PCR).

同时通过突变分析、定量逆转录聚合酶链反应（qRT-PCR）研究了EnvZ/OmpR二元系统在应对渗透压力中的作用。

Methods R and/ or G banding technique was used for analysis of karyotype. DNA blotting for HRX gene rearrangement, and polymerase chain reaction ( PCR) and reverse transcriptase PCR ( RT PCR) for fusion gene detection.

方法用R带和（或）G带分带技术进行核型分析，DNA印迹法检测HRX重排，聚合酶链反应和逆转录聚合酶链反应进行融合基因检测。

Method RhD mRNA was analyzed through reverse transcriptase PCR ( RT-PCR) and cDNA sequencing in 4 individuals with different Rh phenotypes ( CcDDEe 、 CCDDee 、 CcDDee and ccDdee).

方法采用逆转录PCR（RT PCR）技术检测4名不同Rh表型（CcDDEe、CCDDee、CcDDee和ccDdee）个体的RhD mRNA，然后进行cDNA测序分析。

Extraction of total RNA from culture clones which were expanded, and then detecting the target gene was integrated into the genome of HeLa cells through reverse transcriptase PCR method ( RT-PCR).

对单克隆进行扩大培养，提取其总RNA，逆转录酶PCR法（Reverse transcriptase-PCR，RT-PCR）检测到目的基因已整合到HeLa细胞基因组中，说明重组质粒在细胞中稳定表达。

Methods Total RNA in the metastatic lymph nodes from patients with colorectal cancer was extracted routinely, and reverse transcriptase PCR employed to amplify the heavy chain Fd and light chain κ cDNA.

方法取大肠癌病人转移淋巴结，提取淋巴结总RNA，逆转录PCR扩增重链Fd和K轻链cDNA。

After sacrificing the rats in twelve weeks, serum and aorta were studied through the techniques: biochemistry, radioimmunoassay, immuno-histochemistry ( IHC), reverse transcriptase PCR ( RT-PCR) and western blot.

各组大鼠实验12周后处死，提取血清、主动脉、通过生化、放免、免疫组化、RT&PCR法及蛋白印迹杂交法进行研究。

The expression of TLR 4, MD-2 mRNA were assayed by reverse transcriptase polymerase chain reaction ( RT-PCR ).

逆转录聚合酶链反应 ( RT -PCR ) 检测各组细胞TLR4和MD-2mRNA的表达.

C fos gene expression level was semi quantified by reverse transcriptase ( RT) PCR.

采用逆转录（RT）PCR对C Fos基因表达水平进行半定量分析。

Detection for HCV with FD thermostable reverse transcriptase mediated RT nested PCR

FD耐热逆转录酶介导的逆转录nested PCR检测丙型肝炎病毒

In the present study, reverse transcriptase polymerase chain reaction ( RT-PCR) was used to detect the changes of the mRNAs expression of 5-HT_ 2~ 7 receptor subtypes in the rat dorsal root ganglia ( DRG) induced by spared nerve injury ( SNI).

本研究利用反转录聚合酶链式反应（RT-PCR）方法，观察了坐骨神经分支选择性损伤（SNI）所致神经病理性痛条件下，大鼠背根神经节（DRG）中5-HT2~7受体亚型mRNAs的时程表达变化。

Methods: The mRNA was extracted from the fresh tissues of 16 colonic cancer patients; reverse transcriptase polymerase chain reaction ( RT-PCR) was used to detect the receptors of TRAIL.

方法：取16例新鲜结肠癌组织提取mRNA，用逆转录聚合酶链反应（RT-PCR）方法检测TRAIL受体的表达。