affinity chromatography的例句大全

Purified vMIP ⅱ was produced through metal chelated affinity chromatography, enterokinase digestion to free vMIP ⅱ from fusion protein and cation exchange chromatography.

经金属离子亲和吸附、肠激酶消化以游离vMIPⅡ、阳离子交换层析等步骤纯化目的蛋白。

The purification of AFP from the cancered ascites by means of affinity chromatography and the preparation of corresponding monoclonal antibody

从癌性腹水中亲和层析提纯AFP并制备相应的单克隆抗体

Recombinant hKS with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

经肠激酶切割和纯化，获得了与天然蛋白N-末端相同的hK5重组蛋白。

The polypeptide of interest was purified by Ni2+ affinity chromatography under denaturing conditions, followed by renaturation of the recombinant protein by dialysis, and digestion with enterokinase to release the chimeric peptide.

经表达条件优化，大量制备重组蛋白，变性条件下进行镍离子亲和层析纯化。对纯化后的重组蛋白进行复性，用肠激酶切割复性蛋白以获得目的多肽。

Boronate affinity chromatography of antibiotics I. Study on the possibility of complexation of erythromycin and kanamycin with boric acid

抗生素的硼酸基亲和层析I.红霉素和卡那霉素与硼酸络合可能性的研究

immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione ( gsh) using chitosan as substrate.

制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽（GSH）。

Purification by Affinity Chromatography and Characterization of Tea Leaf β-Glucosidase

茶叶β-葡萄糖苷酶亲和层析纯化与性质研究

Methods: These hormones were extracted and purified from cooled human pituitary glands by affinity chromatography 、 gel filtration chromatography and so on.

方法：以冷冻人脑垂体为原料经离子交换层析、凝胶过滤和亲和层析等方法可获得达国际标准品水平的激素纯品。

Isolation and purification of human cardiac troponin ⅰ using affinity chromatography

亲和层析法分离纯化人心肌肌钙蛋白Ⅰ

CALB was purified through Ni-NTA affinity chromatography and ultrafiltration, and was then analyzed by SDS-PAGE. The result showed that the CALB protein expressed by P. pastoris was about 35 kDa, which might contain the glycosylated brach chain.

对经Ni-NTA亲和层析和超滤浓缩纯化的CALB进行SDS-PAGE分析，发现在CalB基因在P.pastoris中表达产物的大小为35kDa（含糖基化侧链）。

Objective A simple method for detection of hepatoma-specific gamma-glutamyltranspeptidase ( HS-GGT) was set up by Datura stramonium agglutinin ( DSA) affinity chromatography, and its clinical application was verified in differential diagnosis of primary hepatocellular carcinoma ( PHC).

目的建立一种特异的检测血清中肝癌特异性γ-谷氨酰转移酶（HS-GGT）的亲和层析方法，并观察HS-GGT对原发性肝癌（PHC）的鉴别诊断价值。

Usually, people apply the thioredoxin fusion system containing the gene IL-11 to express IL-11 fusion protein in the E. coli and apply the immobilized metal affinity chromatography to purify the expression.

一般采用含有IL-11基因的硫氧还蛋白系统在重组大肠杆菌中来表达制备IL-11融合蛋白，产物经金属鏊合层析进行初次分离。

After ultrafiltration and dialysis, protein C was purified further with ion exchange chromatography and immobilized metal affinity chromatography.

超滤透析后，再通过串联离子交换层析和固定化金属螯合亲和层析，对蛋白C进行纯化。

a peptide, luffin p1, from seeds of luffa cylindrica, was purified by ammonia sulfate precipitation, cm-52 ion exchange chromatography, blue-gel affinity chromatography and fplc mono s ion exchange chromatography.

通过硫酸铵分级沉淀、CM52阳离子交换层析、蓝胶亲和层析和FPLC Mono S阳离子交换层析，从丝瓜籽抽提液中分离到一种多肽Luffin P1。

After denature and renature procedure in Ni~ ( 2+)_NTA affinity chromatography column, soluble chitinase was obtained.

在Ni2+-NTA亲和柱上经变、复性和纯化，得到可溶的几丁质酶。

Porcine C1q was purified from porcine serum by means of euglobulin precipitation by PEG, low ionic strength precipitation, IgG-Sepharose 4B affinity chromatography and Sephadex G-200 chromatography.

猪C1q由猪血清通过PEG沉淀优球蛋白、低离子强度透析沉淀、IgG－Sepharose48亲和层析、Sephadex G－200凝胶层析等步骤分离纯化，每300ml血清可制得9.8mgC1q，产率为46.7%。

Study on isolating complement C3d from the chicken serum by the method of immuno affinity chromatography

免疫亲和层析法分离鸡补体C3d蛋白研究

METHODS BALB/ c mice were immunized with human liver microsome proteins, and mAb was prepared by hybridoma technique. The crude mAb was purified by protein-G affinity chromatography.

方法：以含hCE-Ⅱ的人肝脏微粒体蛋白混合抗原免疫BALB/c小鼠，采用杂交瘤技术制备鼠抗hCE-Ⅱ的mAb，并用Protein-G亲和层析法纯化mAb。

Analysis to Fields Synergy for the Affinity Chromatography in a Tapered Bed

锥形床亲和层析过程的场协同分析

affinity chromatography materials for glutamate decarboxylase

谷氨酸脱羧酶亲和层析介质制备