Conclusion An immortalized rat astrocyte strain expressing enkephalin regulated by Dox has been established, which may provide a new tool for regulatable gene therapy for chronic pain in the future.
结论成功构建了可调控的定量表达脑啡肽的永生化星形胶质细胞株,其有望成为慢性疼痛治疗的新方法。
Methods: BK related intracellular calcium changes in astrocyte, C6 glioma cell and brain microvascular endothelial cell ( BMEC) were studied respectively and Western blot analysis of B_2 receptors was performed in the above three lines of cell.
方法免疫荧光测定脑血管内皮细胞、胶质细胞及C6细胞在不同剂量缓激肽作用前后的细胞内钙离子变化,western blot测定3组细胞的缓激肽B2受体表达水平。
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