Methods By using reverse-phase HPLC and fluorescence labeled substrate, the effect of some factors on GnT V activity was investigated, including enzyme protein concentration, incubation time, pH and detergent.
方法采用反相HPLC荧光法测定GnTⅤ活性时的一些影响因素,包括反应时间、酶蛋白量、pH和去垢剂等。
This paper reports the application of fluorescence anisotropy technique to the ( assay) of proteases via a protein substrate labeled with tetramethyl isothiocyanate rhodmine ( TMRITC).
建立了一种基于荧光各向异性原理均相测定蛋白酶及其抑制剂的方法。
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