The osmoregulation proB and proA genes from Bacillus subtilis 93151 are overlapping genes, which encode two proteins ProB and ProA.
枯草芽孢杆菌93151的渗透压调节基因proB和proA以重叠基因的方式组织,但是表达两个单独的蛋白质ProB和ProA。
A restriction enzyme site was inserted in the overlapping region of proB and proA genes from a salt-tolerant mutant of B. subtilis 93151, and a fusion gene was constructed by cloning proB and proA genes respectively.
通过引物设计,在抗脯氨酸反馈抑制耐盐突变菌株9315114的proBA基因重叠区引入一个限制性酶切位点,分别扩增出proB和proA基因,并构建融合的proBA基因。
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