Methods Ames test was conducted with rat liver S9 metabolic activation system ( S9-mix) induced by Compound Danshen Dripping Pills at different doses, and the amounts of reverse mutation induced by 2-AAF were compared between groups with different S9-mix.
方法将复方丹参滴丸不同剂量诱导处理后的大鼠肝S9作为代谢活化系统进行Ames试验,比较加入S9前后2AAF对鼠伤寒沙门氏菌的回复突变率。
Then at the 6 point will lead to a higher pregnancy rate. The reverse mutation frequency of the protoplast of the auxotrophic mutants was less than 10  ̄(-6).
营养缺陷型菌株原生质体回复突变率小于10 ̄(-6)。
Reverse transcription-polymerase chain reaction was employed to evaluate the expression of PRL mRNA in pituitary glands, and DNA sequencing was used to analyze the mutation in regulatory sequence of PRL gene.
应用RT-PCR方法检测PRL基因的表达,同时克隆PRL基因5'上游调控序列-1206~-1966bp片段,采用DNA直接测序方法检测该序列的突变情况。
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