reverse transcriptase的例句大全

Third, for any virus that did enter cells, the microbicide would block an enzyme, reverse transcriptase, that the virus needs to replicate.

第三，对于任何进入细胞的病毒来说，抗生素可以阻断一种病毒赖以复制的逆转录酶。

Objective: To study the human telomerase reverse transcriptase ( hTERT) activity and expression of PTEN protein in squama cell carcinoma of tongue and to investigate their relations during histogenesis and development of squama cell carcinoma of tongue.

目的：探讨舌鳞状细胞癌组织中hTERT与PTEN蛋白表达的相关关系，为治疗原发性舌鳞癌提供理论依据。

Method The rotavirus genome in the plasma samples of 60 infants with rotavirus diarrhea and in the peripherial blood mononuclear cells ( PBMCs) of 14 out of the infants who showed abnormal serum enzyme levels were subjected to nest reverse transcriptase polymerase chain reaction test.

方法应用套式一逆转录PCR方法检测60例RV腹泻患儿外周血血浆和其中14例血清酶学异常患儿外用血单个核细胞中的RV。

Moreover, the detection limit had been improved by the use of Superscript reverse transcriptase.

并且，使用了Superscript反转录酶进行反转录，提高了检测的灵敏度。

Expression of Human Telomerase Reverse Transcriptase and in Genitalia Leukoplakia and Squamous Cell Carcinoma of Vulva

人端粒酶逆转录酶在外阴白斑和外阴鳞癌中的表达

Methods Gene expression associated with vascular channel in osteosarcoma endothelial cell was measured by semi-quantitative reverse transcriptase polymerase chain reaction using the transcriptional level of the house-keeping gene for internal calibration.

方法利用RT-PCR技术对骨肉瘤肿瘤细胞（MG63、U2OS）血管形成相关基因进行检测，并同内皮细胞（ECV304）相比较。

Extraction of total RNA from culture clones which were expanded, and then detecting the target gene was integrated into the genome of HeLa cells through reverse transcriptase PCR method ( RT-PCR).

对单克隆进行扩大培养，提取其总RNA，逆转录酶PCR法（Reverse transcriptase-PCR，RT-PCR）检测到目的基因已整合到HeLa细胞基因组中，说明重组质粒在细胞中稳定表达。

Methods Total RNA in the metastatic lymph nodes from patients with colorectal cancer was extracted routinely, and reverse transcriptase PCR employed to amplify the heavy chain Fd and light chain κ cDNA.

方法取大肠癌病人转移淋巴结，提取淋巴结总RNA，逆转录PCR扩增重链Fd和K轻链cDNA。

After sacrificing the rats in twelve weeks, serum and aorta were studied through the techniques: biochemistry, radioimmunoassay, immuno-histochemistry ( IHC), reverse transcriptase PCR ( RT-PCR) and western blot.

各组大鼠实验12周后处死，提取血清、主动脉、通过生化、放免、免疫组化、RT&PCR法及蛋白印迹杂交法进行研究。

Objective To establish polymerase chain reaction ( PCR) and reverse transcriptase PCR ( RT-PCR) for detecting human cytomegalovirus ( HCMV) and to find out the relationship between diabetes mellitus ( DM) and HCMV infection.

目的建立检测人巨细胞病毒（HCMV）感染的聚合酶链反应/逆转录&聚合酶链反应（PCR/RT-PCR）方法，并探讨糖尿病与HCMV感染的关系。

Method RhD mRNA was analyzed through reverse transcriptase PCR ( RT-PCR) and cDNA sequencing in 4 individuals with different Rh phenotypes ( CcDDEe 、 CCDDee 、 CcDDee and ccDdee).

方法采用逆转录PCR（RT PCR）技术检测4名不同Rh表型（CcDDEe、CCDDee、CcDDee和ccDdee）个体的RhD mRNA，然后进行cDNA测序分析。

C fos gene expression level was semi quantified by reverse transcriptase ( RT) PCR.

采用逆转录（RT）PCR对C Fos基因表达水平进行半定量分析。

Detection for HCV with FD thermostable reverse transcriptase mediated RT nested PCR

FD耐热逆转录酶介导的逆转录nested PCR检测丙型肝炎病毒

The replication of HBV DNA passes through reverse transcription. As the reverse transcriptase is lack of effective function of proofreading the bases, the HBV genome is easier to mutate than other DNA viruses.

HBV复制过程中要经过逆转录，由于逆转录酶缺乏有效的碱基校对功能，故HBV基因组比一般DNA病毒易发生变异。

Research Progress in Targeting Human Telomerase Reverse Transcriptase ( hTERT) Promoter in Cancer Gene Therapy

靶向人端粒酶逆转录酶启动子在肿瘤基因治疗中的研究进展

In the present study, reverse transcriptase polymerase chain reaction ( RT-PCR) was used to detect the changes of the mRNAs expression of 5-HT_ 2~ 7 receptor subtypes in the rat dorsal root ganglia ( DRG) induced by spared nerve injury ( SNI).

本研究利用反转录聚合酶链式反应（RT-PCR）方法，观察了坐骨神经分支选择性损伤（SNI）所致神经病理性痛条件下，大鼠背根神经节（DRG）中5-HT2~7受体亚型mRNAs的时程表达变化。

A Theoretical Study of Non-nucleoside Inhibitor MKC Molecular Binding Sites at the HIV-1 Reverse Transcriptase

HIV-1逆转录酶非核苷类抑制剂MKC分子结合位点的理论研究

Reverse transcriptase Detection of BCR-ABL fusion gene in Ph1 chromo-some positive leukemia by nested

用筑巢式逆转录酶／多聚酶链反应检测Ph1阳性白血病特异基因转录本

Detection of Hantavirus by reverse transcriptase semi nested polymerase chain reaction and its preliminary application

逆转录-半套式聚合酶链反应检测汉坦病毒RNA及其临床初步应用

Expression of Telomerase RNA and Telomerase Reverse Transcriptase mRNA in Lesions of Psoriasis Vulgaris

寻常性银屑病皮损端粒酶RNA、反转录酶mRNA表达