microinjection

To the great extent, the study and application of transgenic animal are restricted by the inherent limitation of pronuclear microinjection.

显微注射技术本身固有的缺点在很大程度上限制了转基因动物的研究及应用。

Microinjection of methlysergide ( MS), an antagonist of 5-HT receptor, into the hippocampus, had no direct effect on sleep, but abolished the SWS-promoting effect of 5-HTP completely.

非特异性5-HT受体阻断剂麦角新碱（MS）对睡眠无直接影响，但可完全阻断5-HTP的促睡眠效应。

Then we transport reporter gene driven by CMV promoter and erythrocyte-specific GATA-1 promoter into the embryos via microinjection approach and observed the spatial and temporal expression pattern of EGFP.

分别将病毒启动子CMV和斑马鱼血细胞特异性表达基因GATA-1的启动子驱动的报告基因EGFP表达载体通过显微注射转入斑马鱼胚胎，观察了报告基因EGFP的表达情况。

An ion current expression after intracellular microinjection of mRNA in the Xenopus oocytes

卵胞内微注射mRNA后胞膜上出现的新离子流

The pain threshold was not influenced by microinjection of lidocaine into NRM or RN.

单独用利多卡因阻断双侧红核或中缝大核对痛阈无影响.

Study on Techniques of Making Transgenic Mice by Microinjection Method

显微注射法制备转基因小鼠的技术研究

Persistent injection of Morphin to epidural space by microinjection pump to treat late-stage cancer pain

微量泵持续硬膜外腔注射吗啡治疗晚期癌痛

Whole mount in situ hybridization and transgene via microinjection are two important methods in zebrafish study.

整胚原位杂交和显微注射转基因技术是斑马鱼基因功能研究中两种重要的研究方法.

Research on Microinjection in Transgenic Microoperation System

转基因超微量注射控制器的研究

Microinjection, retrovirus mediated method and embryonic stem cells method were all the traditional methods of producing transgenic animals. But these methods had defects and limited application in the research of transgenic animals in the future.

传统的制作转基因动物方法有显微注射法、逆转录病毒感染法和胚胎干细胞法等，但每种方法都有其缺陷，限制了其在今后转基因动物研究中的广泛应用。

Study on the Technology of Microinjection and Binocular Time-Division Three-Dimensional Imaging in Transgenic Microoperation System

转基因微细作业系统中超微量注射及双目时分立体成像技术的研究

To construct a murine model that is immunotolerant to HBsAg, we developed the HBsAg ( ayw subtype) transgenic ( Tg) mice with CMV promoter by microinjection.

为建立对HBsAg耐受的小鼠模型，利用显微注射转基因技术，构建了CMV启动子控制的HBsAg（ayw亚型）转基因小鼠。

The effect of intracerebroventricular microinjection of 0.1 μ g pentagastrin ( G-5) on rat's gastris electricity and gastric movement were observed.

在大鼠侧脑室微量注入五肽胃泌素（G-5）0.1μg观察其对胃电和胃运动的影响。

Changes of Sleep& wakefulness Induced by Microinjection of Bromocriptine Into the Ventral Tegmental Area in Rats

VTA微量注射溴隐亭诱发大鼠睡眠&觉醒的改变

The Effect of Pronuclear Microinjection with Foreign DNA and Culture Conditions on the Development in vitro of Mouse Pronuclear Embryos

影响小鼠转基因原核胚体外发育的若干因素

A very small pipette used in microinjection.

一种小型的或年幼的隼.

Five transgenic positive mice were obtained through pronuclear microinjection.

通过原核注射，获得5只转基因阳性鼠；

METHODS: Transgenic mice were produced by pronu-clei microinjection method.

方法：原核显微注射法产生转基因小鼠。

Mathematical model of microinjection amount is founded through depth theory research to the microinjector.

本文通过对显微注射仪的深入研究,建立了注射量的数学模型.

Effect of microinjection of etorphine into dorsal hippocampus on forelimb withdraw reaction induced by stimulating splanchnic nerve in conscious rabbit

海马背部微量注射埃托啡对家兔内脏大神经伤害性刺激引起回缩反应的影响

Methods We isolated embryonic stem cells from inner cell mass of KM mice blastocysts. Then we transferred embryonic stem cells into blastocoele of C57BL/ 6J inbred mice by microinjection in order to construct chimeric mice.

方法从KM小鼠囊胚的内细胞团中分离培养胚胎干细胞，进行近交系C57BL6J小鼠受体囊胚腔内注射，进行嵌合体构建实验。

Effect of intracerebroventricular microinjection of caerulein on gastric juice secretion and content of PGE_2 in gastric juice of rats

侧脑室内微量注射雨蛙肽对大鼠胃液分泌及胃液中前列腺素E2含量的影响

The basic reliable methods available to produce transgenic animal such as pronuclear microinjection has limited application by low efficiency and mosaic integration into the genome.

由于生产转基因动物的基本方法如原核注射法等普遍存在外源基因整合的低效性和随机性等问题，因而在应用中受到很大制约。

In addition , the evoked responses could be inhibited by intralateroventricular microinjection of atropine.

侧脑室注射阿托品抑制扣带回前部神经元内脏痛觉诱发反应.

Furthermore, combining information of our research , it is emphatically introduced about microinjection into chicken , s blastoderm.

其中, 结合本实验室的情况, 重点介绍了胚盘直接注射法.

By experimental confirmation, the automatic cell injection is scheme, which is based on cell pressure regulation, cell auto-transmission, micro-needle automatic positioning, and quantitative microinjection.

结果表明：基于细胞压力调节、自动输送、微针自动定位技术和定量微注射的自动化细胞注射是可行的。

Microinjection of MK-801 into ventral tegmental area reduces naloxone-precipitated morphine withdrawal in rats

腹侧被盖区微注射MK-801对大鼠吗啡戒断症状的影响

Models of adjuvant arthritis ( AA) in rats were made, and microinjection of 6-hydroxydopamine ( 6-OHDA) into hippocampus was given, and then changes in vola circumference, index of thymus and spleen, IL-6, IL-2 after moxibustion were observed.

实验建立AA大鼠模型，应用6OHDA作海马内微量注射，观察灸疗对跖围、胸和脾指数、IL6、IL2等炎症和免疫指标的改变。

Furthermore, with only a half dose of DNA in the current system, by the horizontal system under × 100 magnification both the survival rate of pronuclear embryos following microinjection and the integration frequency of exogenous DNA injected into embryos also increased.

此外，利用该系统还可在低倍镜下（×100）将DNA常规注射量减半注射，提高了原核胚注射后的成活率，其注射胚的外源基因整合率亦明显提高。

Methods Alkaline lysis assay was used to extract and purify the plasmid. Plasmid DNA was injected into tumor by microinjection assay.

方法：碱裂解法提取纯化质粒DNA，微量注射法直接将质粒DNA导入体内肿瘤；