plasmids

Multidrug resistance is frequently linked with DNA elements such as integrons, which can be located on transposons and plasmids but also on the chromosome. They can integrate and disseminate genes encoding resistance to a number of antimicrobial drugs.

多重耐药细菌往往携带诸如整合子等基因元件。整合子位于质粒、转座子和基因组上，它们可以整合并且传播大量的耐药基因。

The plasmids were extracted by alkaline lysis technique.

应用碱裂解法提取相应菌株的质粒，对质粒谱进行分型。

The key to this riddle appeal to be plasmids, which can block phage adsorption.

这个难题的关键是质粒, 它能够阻碍噬菌体的吸附作用.

New phages can develop by acquiring restriction enzymes from plasmids.

通过从质粒获得的限制性酶,新的噬菌体能够生长.

Finally, alkaline lysis and anion exchange chromatography were used to release and purify plasmids.

最后用碱裂解法裂解细胞提取质粒，并将提取物进行阴离子交换层析以进一步纯化质粒。

Plasmids in Parenthesis are in free state, plasmids after diagonal line are in intergrated state.

在括号中的质粒表示质粒处在自主状态, 斜线后的质粒表示质粒处在整合状态.

Plasmids are exta-chromosomal doublestranded, circular DNA molecules found in prokaryotic and eukaryotic cells.

质粒是在原核和真核细胞中发现的染色体外的双链环状DNA分子.

Objective To investigate the amelogenin expression of recombinant plasmids PsecTaq 2 A-AMG in COS 1-cell line.

目的研究人釉原蛋白重组质粒PsecTaq2A-AMG在COS -1 细胞系中的表达,为基因工程制备釉原蛋白奠定基础.

Many of the currently known resistance genes are located on mobile genetic elements such as plasmids, transposons, integrons and genomic islands.

已知的许多耐药基因大多位于可移动元件如质粒、转座子、整合子和基因岛上。

Results The proportion of G-bacilli were much. Study on Plasmids of Enteric Pathogens

结果G-杆菌比例居多，条件致病菌和酵母样真菌检出机会显著增高；肠道病原菌的质粒研究

The plant is then transformed using the engineered Agrobacterium containing both plasmids, one including the VIR region and the other the T-DNA.

于是就可以用含有两个质粒的工程农杆菌转化植物，即工程农杆菌的一个质粒含有VIR区，另外一个质粒含有T-DNA。

Antibiotic resistance gene have frequently been found to be encoded by determinants carried on mobile genetic elements, such as plasmids, transposons, and integrons, which are then responsible for the horizontal transfer of antibiotic resistance genes.

细菌中可移动遗传单位如：质粒、转座子和整合子可以携带编码耐药基因的基因盒。整合子是耐药基因水平转移的主要因子。

Table 1 Important plasmids and strains used in this study.

1质粒和菌株.实验中用的关键质粒和菌株见表1.

Insertional mutagenesis using T-DNA, transposons and plasmids are commonly methods to creating a mutant library.

构建插入突变库的方法有T-DNA插入突变、转座子插入突变和质粒介导的插入突变。

Recombinant ( SFV) and plasmids were constructed and identified for their expression of MAGE-3 in vitro.

构建重组病毒和重组质粒并在体外转染鉴定其表达。

Based on the analysis of cytoplasmic like-plasmid, WB-a new male sterile cytoplasm identified recently by Huazhong Agricultural University was found to contain S_1 and S_2-type plasmids. The result indicated that WB was further confirmed belonging to S-group cytoplasmic male sterility.

通过线粒体类质粒分析，表明华中农业大学发现的新雄性不育胞质WB带有S组特有的S1、S2类质粒，据此将WB确认为S组雄性不育胞质。

In addition, constructed plasmids can be by gap repair of this technology.

此外可运用该技术中缺口修复的方法构建质粒.

Cloning of Chicken CD4 and CD8 α cDNA and Construction of Their Eukaryotic Expression Plasmids

鸡CD4和CD8α基因cDNA的克隆及其真核表达质粒的构建

Results The structures of recombinant plasmids were comfinaed by PCR and sequencing.

结果重组体经PCR和测序证实准确连接.

DNA computing model and algorithm for the minimal covering problems by plasmids were presented.

给出了图的最小顶点覆盖问题的质粒DNA计算模型及其实现算法.

We are also studying how the linear chromosomes and plasmids are transferred during conjugation.

此外我们也在研究线状的染色体与线状质体在接合生殖过程中如何地传送.

Conclusion The prepared magnetic nanoparticles coupled with DEAE can be used to purify high purity plasmids.

结论在纳米磁性粒子外包裹上DEAE基团，可以提纯出高纯度的质粒。

RESULTS: The three plasmids were effectively transferred into 293 T c ells.

结果: 表达慢病毒的3种质粒可高效转染入293T细胞.

The Effects of Endogenous Photodynamic Therapy on Human Colon Carcinoma Cell Transfected with Plasmids Containing Cytosine Deaminase Gene and G_1 Phase Cell Cycle Checkpoints

内源性光动力学结合CD基因转染增强杀伤结肠癌细胞作用以及对细胞G1期关卡因子的影响以及不确定性来源的内生性等问题。内源性光动力疗法抑制人结肠癌裸鼠种植瘤的实验研究

The stronger fluorescence signals were detected in transfected HEK293 cells with these two kinds of plasmids by immunofluorescence assay.

转染后免疫荧光技术检测显示两者皆可在HEK293细胞中正确表达。

Objective To explore the role of plasmids in inducing drug-resistance in Neisseria gonorrhoeae.

目的探讨质粒在介导淋球菌耐药中的作用.

Coexpression of caiB and caiE with Incompatible Plasmids in E.coli

不相容双质粒共表达大肠杆菌肉碱脱水酶基因caiB及其辅因子合成酶基因caiE

Objective To explore the pilot fermentation procedure of recombinant E. coli strain containing two plasmids as therapeutic HBV DNA vaccine.

目的研究双质粒HBV dna疫苗工程菌的中试发酵工艺。

After plasmid purification, the acquired recombinant plasmids were transfected into BHK-21 cell with liposome mediated method. the expression of P32 and CD58 were tested by direct immunofluorescence assay ( IFA) or indirect immunofluorescence assay.

质粒提纯后，在脂质体作用下转染BHK-21细胞，通过直接免疫荧光试验和间接免疫荧光试验分别检测P32和CD58在BHK-21中的表达。

The four plasmids containing different length of RNA polymerase gene of poliovirus were constructed to express RNA polymerase.

将含脊髓灰质炎病毒（PV）RNA聚合酶的不同长度基因片段克隆到载体pSG5质粒上，分别构建了4个表达RNA聚合酶的质粒。