primer RNA

Then, RT-PCR was carried out for IL-10 gene screening using total RNA as a template, specific primers which derived from GenBank data of hIL-10 as a primer for reverse transcription and PCR primers.

以总RNA为模板，根据人IL-10在GenBank中的全长开放读框设计的特异性引物进行反转录，RT-PCR进行IL-10基因筛选。PCR产物经酶切分析和DNA序列测定后；

Methods: Total RNA of new born rat liver was extracted as a template, first strand of cDNA was synthesized by reverse transcription with olig ( dT)-primer and then amplified by PCR with the primers designed by ourselves.

方法：提取新生大鼠肝脏总RNA为模板，以寡聚dT为引物逆转录合成第一链cDNA，再以我们设计的PCR引物进行PCR扩增获取大鼠肝再生增强因子编码区双链cDNA；