promoter site

Conclusion The recombined mammalian expression vector, containing both G6p promoter and site-mutated proinsulin, is a novel ideal candidate vector for diabetic gene therapy.

结论带有G6p启动子的含定点突变胰岛素原基因的真核表达载体有望成为糖尿病基因治疗理想的候选载体。

By artificially changing A to C at-137 bp site upstream from transcription start point of cloned promoter, two site-mutation promoters, IPM s ( 603 bp) and IPM 1 ( 900 bp) were created.

通过PCR方法对克隆的两个启动子进行定点突变，使转录起始位点上游-137bp处A突变为C，得到两个突变启动子（IPMl、IPMs）。

Moreover, results of the site-directed mutagenesis of a C-repeat/ DRE and two APE-like motifs in a promoter-like region(– 630/ – 308) suggested that two APE-like motifs might be essential for transcriptional regulation of the bkt gene.

对其启动子类似区（-630/-308）中两种功能元件的定点突变结果发现APE-like（蓝光调控元件）对于bkt基因自身的转录可能存在影响。

Construction of bsh Promoter Mutants by Rapid One-step Site-directed Mutagenesis

一步法定点突变技术快速构建bsh基因突变启动子

And a cryptic start site of transcription was found, which was located upstream of the internal promoter TATA box and used in the natural infection.

并发现在BFV3026的内部启动子近上游存在神秘转录起始点，该起始点在泡沫病毒的自然感染进程中发挥作用。