recombinant plasmid

Construction of recombinant plasmid highly expressing luciferase in Drosophila S2 cells

果蝇S2细胞中高表达荧光素酶重组质粒的构建及活性测定

Construction of Recombinant Plasmid Containing Snake Venom Cystatin Gene and Its Expression

蛇毒cystatin基因真核表达质粒的构建与表达

Mice bilateral tibialis anterior muscle were immunized by recombinant plasmid via electrical impulse. The mice were infected with Salmonella typhimurium after four times. Mice body temperature and the number of leukocyte, neutrophilic granulocyte and lymphocyte number were detected after four weeks.

利用电脉冲介导，将重组质粒免疫小鼠双侧胫骨前肌，然后用鼠伤寒沙门氏菌进行攻毒，测定小鼠体温及血液中的部分免疫指标：白细胞数、中性粒细胞数和淋巴细胞数等。

DNA sequencing for the recombinant plasmid demonstrated the obtained fragment are completely identic with previous expected design.

经DNA测序，证明重组质粒序列完全符合预期设计。

Objective To investigate the amelogenin expression of recombinant plasmid PcDNA 3-AMG in COS-1 cell line.

目的研究人釉原蛋白(AMG) 重组质粒PcDNA3-AMG在COS -1 细胞系中的表达.

The relationship between template activity and structural variation of recombinant plasmid DNA was studied in a cell-free system for in vitro transcription which was developed with maize RNA polymerase B and recombinant plasmid DNA.

本文以重组质粒pZmS51DNA为转录模板，在无细胞离体转录系统中研究了质粒DNA的构型变化与其模板活性的关系。

Methods: Porin B of gonorrhoeae expressed in E. coli by a recombinant plasmid and purified, were administered subcutaneously into the rabbit back. The serum antibody change and the highest titer of the rabbit was dynamically examined after immunized by ELISA.

方法：兔背皮下注射经原核构建表达并纯化的淋球菌孔蛋白B，ELISA动态检测免疫后大耳兔血清抗体滴度的变化及最高效价；

Construction of LACK Gene Recombinant Plasmid and Detection of Its Expression in Eukaryotic Cell

杜氏利什曼原虫LACK基因真核表达重组质粒及其在真核细胞中的表达

Objective Construct a recombinant plasmid pET 28 a-EDA-EDB , prepare the fusion EDA-EDB protein.

目的构建重组pET28a-EDA-EDB 质粒, 制备重组纤维连接蛋白EDA -EDB 融合蛋白.

Studies on the stability of recombinant plasmid in groundnut Rhizobium

重组质粒在花生根瘤菌中的稳定性

Construction and Sequence Analysis of A Recombinant Plasmid Harbouring the Porin Gene PIA of Neisseria gonorrhoeae

淋球菌主要外膜蛋白PIA原核重组质粒的构建及序列分析

Results The recombinant plasmid pET28a (+) PIA was constructed successfully.

结果成功构建PIA基因原核重组质粒pET28a（+）-PIA。

Construction of a Recombinant Plasmid Containing Multi-copy CpG Motifs and Its Immuno-modulating Effects on the Immune Responses of Aquatic Animals, Fish and Prawn

一种含多拷贝CpG基序的质粒构建及其对水产动物的免疫调节作用

Characterization Analysis of Recombinant Plasmid pZ-55 Containing hup Genes from Rhizobium arachis

含花生根瘤菌吸氢基因重组质粒pZ-55的特性分析

Conclusion The bivalent recombinant baculovirus transfer plasmid will be applied to the constuction of bivalent L1 protein expression system, then contributed to the establishment the bivalent gene engineering subunit vaccine.

结论本研究成功构建了双价重组杆状病毒转移质粒为进一步构建双价L1蛋白表达系统进而建立双价基因工程亚单位疫苗打下基础。

The recombinant CYP 3 A 4 plasmid was successfully cloned and effectively expressed in Rosetta ( DE 3 ) 2 pLysS.

成功地使CYP3A4基因在原核系统中高效表达.

A recombinant plasmid pUC-IL 6 coding for IL 6 was constructed by recombinant gene technique.

利用基因重组技术,构建含IL6基因的重组质粒pUC - IL6.

Construction of Fusion Recombinant Plasmid Coding for RGD Peptide and Urokinase B Chain, Its Expression in Escherichia coli and Preliminary Characterization of Its Biological Activity

RGD肽与尿激酶B链融合蛋白原核重组表达质粒的构建、表达和功能的初步分析

Objective To select an optimal combined set of carrier, introducing route and dose of recombinant plasmid of augmenter of liver regeneration ( ALR) for ALR gene therapy.

目的筛选肝再生增强因子重组质粒基因治疗时较优的载体、途径和剂量及三者的最佳组合。

Structural Variation of Recombinant Plasmid DNA and It's Regulation on Transcription

质粒DNA的构型变化对转录的调控作用

Construction of the recombinant plasmid pBI GFP

带有绿色荧光蛋白基因（gfp）的植物重组表达质粒pBI-GFP的构建

Objective To construct a recombinant plasmid harbouring the PIA gene of Neisseria gonorrhoeae.

目的构建携带淋球菌主要外膜蛋白PIA基因的pET重组质粒，分析其编码序列。

Methods: Recombinant plasmid DNA of Hendra virus G gene were amplified by conventional PCR and real-time PCR assays, and sensitivity of the two methods was compared.

方法：采用常规PCR法和实时PCR法分别对构建的Hendra病毒全长G基因的重组质粒DNA进行扩增，并比较两种方法的敏感性。

Conclusion: The recombinant plasmid Pw 2 encoding Pw specific antigen was obtained successfully prepared.

结论：成功构建了编码卫氏并殖吸虫特异性抗原的重组克隆Pw-2。

Results A recombinant plasmid pBV/ IL-2/ PEA was constructed.

结果经鉴定，获得重组质粒pBV／IL－2／PEA。

Construction and Identification on Expression Recombinant Plasmid of MxA Gene Eukarya Vector in Horse

马MxA基因真核载体表达重组质粒的构建与鉴定

Conclusion The recombinant plasmid was constructed successfully, and it actually can block the expression of TITIN.

结论pSITITIN具有确切阻断肌联蛋白表达的效果。

The recombinant leptin siRNAs plasmid vectors could be expressed in eukaryocyte, and then to evaluated them by using enzyme cutting and sequencing.

构建能在真核细胞中表达的靶向瘦素基因的siRNAs重组质粒，用酶切方法和测序法对重组体进行鉴定。

After the reading frame was confirmed correct by sequencing, the recombinant plasmid was transformed into expression host E.

测序表明读码框架正确后，将重组子分别转入表达宿主菌E。

Construction of Eukaryotic Expression Recombinant Plasmid of Swine Ghrelin Gene and Histogenic Immunity Examination in Mice

猪Ghrelin基因真核表达重组质粒的构建及免疫小鼠试验