renaturation

The experimental conditions, including temperature, pH, denatured protein concentration and urea concentration, in the renaturation process of recombinant human interferon-γ inclusion body by dilution were studied and at the same time pulse renaturation was tested. The optimum renaturation conditions were obtained.

采用稀释法研究了重组人γ干扰素包涵体复性条件，如温度、pH值、蛋白浓度、复性液中脲浓度，同时对加样操作方式进行了考察，得到了较为适宜的复性条件。

Cloning and Expression of the Thermolabile Hemolysin Gene of Vibrio Parahaemolyticus, Study on Renaturation and Functions of Its Expression Protein

副溶血弧菌不耐热溶血毒素基因的克隆、表达及表达蛋白的复性和功能的研究

A heterozygous sample after denaturation, renaturation of PCR, contains four duplexes: two homoduplexes and two heteroduplexes.

杂合子样品经过PCR变性、复性之后，其组分包含四种双链分子：两种同源双链、两种异源双链。

Renaturation and Mass Spectrometric Analysis of Chemically Synthesized ω-conotoxin M ⅶ A

化学合成ω-芋螺毒素MⅦA的复性与质谱分析

The polypeptide of interest was purified by Ni2+ affinity chromatography under denaturing conditions, followed by renaturation of the recombinant protein by dialysis, and digestion with enterokinase to release the chimeric peptide.

经表达条件优化，大量制备重组蛋白，变性条件下进行镍离子亲和层析纯化。对纯化后的重组蛋白进行复性，用肠激酶切割复性蛋白以获得目的多肽。

Renaturation of high concentration lysozyme using gel filtration chromatography assisted by artificial chaperones

人工伴侣与凝胶过滤色谱耦合作用促进高浓度溶菌酶复性研究

After purification with the column of Ni2+-NTA and renaturation.

利用Ni2+-NTA柱纯化和复性。

Effect of Induction Time on Quality and Renaturation of Inclusion Bodies with Procarboxypeptidase B

诱导时机对羧肽酶原B包涵体质量和复性率影响的研究

The conditions of purification, denaturation and renaturation of recombinant Bovine growth hormone ( BGH) were researched in order to get a good renaturation result.

为了取得较好的复性效果，对重组牛生长激素的纯化及变复性条件进行了研究。

The means of chromatographic renaturation can renature and purify the protein at the same time, thus simplify the procedure of operation and promote the recovery ratio of the product.

色谱复性使蛋白质的复性与纯化同步进行，简化了操作程序，提高了产品的回收率。

After purification, the target protein further renaturation 2-BMP via degeneration dissolving, load dialysis bag.

纯化后的的目的蛋白进一步复性,BMP-2经变性液溶解, 装入透析袋内.

After renaturation, AIF protein binded DNA and induced nuclear apoptosis.

经EMSA与 Hoechst33258 染色实验证实:获得的AIF蛋白具有良好地与DNA结合并可诱导肿瘤细胞核凋亡的能力,凋亡率为37%.

And also the presence of DTT in renaturation solution maybe had no influence on the lysozyme refolding yields.

复性液中DTT的存在对复性效果没有影响。