selective culture

Monocotyledonous expression vector of double T-DNA was constructed with selective marker gene hyg ( hygromycin resistance) in one T-DNA structural domain and insect resistant gene in another T-DNA domain. Agrobacterium strain was transformed with this vector and used to transform embryonic callus of maize by co-culture.

另一个T-DNA结构域含有抗虫基因的双T-DNA单子叶植物表达载体，用以转化农杆菌菌株，再通过共培养转化玉米胚性愈伤组织。

And they are introduced as follows: utilizing of selective culture media, report gene, gene probe and PCR, immune detection, etc., belong to culturable methods;

一是基于培养的方法，包括利用选择性培养技术，报告基因，基因探针和PCR，免疫监测等；

Pig blie salts is the sodium salt of a conjugated bile acid made from pig bile, often used in bacteriological culture media as a selective inhibitory agent.

性状：本品系由猪胆汁精制而成的一种胆汁酸钠盐，是微生物培养基中常用的选择性抑制剂。

Method Contents of N, P and K in tissue culture medium of test-tube plantlet of rehmannia glutinosa were determined by the methods of salicylic acid nitrofication process, molybdenum blue method and ion selective electrode method, respectively.

方法稀释不同倍数MS培养液模拟不同培养时间的地黄试管苗组织培养基，分别用水杨酸硝化法、钼蓝法、离子选择电极法测定N，P，K元素的含量。

In this paper, the effects of three carbon sources, namely, xylose and xylo-oligosaccharides, on the enzymatic selective hydrolysis of plant hemicelluloses, by the anaerobic culture of bifidobacterium adolescentis in vitro, and the abilit to promote the proliferation of bifidobacteria were examined.

通过体外严格厌氧培养技术，比较葡萄糖、木糖和定向酶解植物半纤维素制备的低聚木糖三种碳源增殖青春双歧杆菌的效果，检测了该低聚木糖的双歧因子作用。