semi-lethal gene

A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee ( Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.

基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因，建立该病毒的半套式RT-PCR检测方法。

Two serial specific primers ( P1/ P2, P2/ P3) were used in semi nested PCR, to amplify predicated gene, which is more sensitive and specific than conventional PCR.

半套式PCR是采用一对半特异性引物对目的基因进行扩增的一种方法，该方法较常规PCR的更敏感和更特异。

Application of semi-quantitative polymerase chain reaction in the PCR-RFLP analysis to detect gene polymorphism

半定量聚合酶链反应在PCR-RFLP酶切法测定基因多态性中的应用

Semi-quantitative RT-PCR method and its application in the study on gene expression

半定量RT－PCR方法在基因表达研究中的应用

Semi-quantitative RT-PCR showed that this gene was highly expressed in midgut, low-expressed in fat body and gonad, and not expressed in head.

半定量RT-PCR分析表明，该基因在中肠组织中表达量最高，在脂肪体和生殖腺中表达量较低，在头部则不表达。