serum medium

Method To take sciatic nerves in 3-4 days SD rats and purify Schwann cells. A group: DMEM/ F12 contained 10% calf blood serum. B group: conditioned medium of macrophages activated by self-neural homogenate.

方法取3-4dSD乳鼠坐骨神经，纯化培养许旺细胞，A组加入含10%胎牛血清的DMEM/F12培养基，B组加入自体神经匀浆激活的巨噬细胞条件培养基；

OEG proliferated quickly by free serum medium, and cell count increased by 16 times of primary culture through 9 days cultivation. Its purity is 85%.

通过无血清纯化培养，OEG增殖迅速，原代培养9天，细胞数目可达16倍以上，纯度可达85%。

Cell culture: The primary OEG was cultivated with the olfactory bulb of Wistar neonate rats, and then the purification was done by free serum medium.

细胞培养：新生Wistar大鼠嗅球（OB）做OEG原代培养，然后利用无血清培养基添加一些促进生长的微量物质做纯化培养，为OEG提供了合适的生长环境而大量增殖。

This procedure using antimitotic medium combining with serum free medium could eliminate fibroblasts effectively, injure less Schwann cells, and yield up to 98% purified Schwann cells.

这种抗有丝分裂法结合无血清培养法，既能有效去除成纤维细胞，又能减少对SC的损伤，获得的SC纯净度可达98%。

Umbilical artery rings and umbilical vein rings embedded in three-dimensional collagen gels in DMEM/ F12 ( 1:1) serum-free medium grew well. The new outgrows were positive by CD34 immunohistochemistry.

脐动脉环和脐静脉环在胶原凝胶和DMEM/F12（1：1）无血清培养基中生长良好，CD34免疫组织化学鉴定其为新生的血管。

DESIGN: The cells cultured in 10% serum medium served as negative control group in vitro.

设计：以体外10%血清正常培养的细胞作为阴性对照的实验对照研究。

Induced differentiation of neural crest cells to glial cells in serum medium in vitro

血清对神经嵴细胞向神经胶质细胞分化诱导的体外研究

Conclusion: Human umbilical vessel ring culture model of three dimensions in DMEM/ F12 ( 1:1) serum-free medium has been established.

结论：用无血清培养基和Ⅰ型胶原可以立体培养脐血管环。

Methods Allergic contact dermitis mice models were divided into model group, control group, large, medium, and small doses groups, and blank group. The changes of serum IL-2 、 TNF-α and histological pathogenic change before and after treatment were observed.

方法采用变应性接触性皮炎小鼠模型，分为模型组，对照组及大、中、小剂量组和空白组，观察各组治疗前后血清IL-2、TNF-α的变化。

BBG low, medium and high dose groups compared with model group, serum calcium levels increased, alkaline phosphatase decreased with the model group, in which middle and high dose groups were significantly different ( P0.05, P 0.01).

温肾止痛颗粒低、中、高剂量组血钙含量与模型组相比增加，碱性磷酸酶含量与模型组比较有所降低，其中中、高剂量组具有显著差异（P0.05，P0.01）。

Methods The tissues of nucleus of inferior colliculus in newborn mice were isolated, collected and cultured with condition serum-free medium ( DMEM/ F12, B27, EGF 20 ng/ ml and bFGF 20 ng/ ml) in vitro.

方法分离、收集新生昆明小鼠下丘核区脑组织，体外经EGF和bFGF刺激下培养；