step chromatography

Results Galangin was obtained step column chromatography of polyamide only once.

结果运用聚酰胺柱层析一次分离,获得有效成分高良姜素.

Coupling of the strong cation-exchange ( SCX) with capillary reversed-phase liquid chromatography ( cRPLC) is described for proteomics of rat liver. A step gradient was applied for SCX column, and a linear gradient was applied for RPLC.

以离子交换色谱（SCX）为第一维，反相液相色谱（RPLC）为第二维，对鼠肝组织的蛋白质组进行了研究。

One step Purification of Tissue type Plasminogen Activator Derived from Expression by Milk Gland of Transgenic Goat Using Affinity Chromatography

应用一步亲和层析法纯化转基因山羊乳腺定位表达的组织型纤维蛋白溶酶原激活剂

Δ NSak was purified by two step ion exchange chromatography. The purity was over 95% and the specific activity of Δ NSak was 9 × 10 4 IU/ mg.

工程菌发酵、压榨后，经两步离子交换层析分离纯化，可获纯度达95%以上、比活性为9.0×104IU／mg的ΔNSak，与野生型Sak相似。

Results Galangin was obtained by on-step column chromatography of polyamide only once.

结果运用聚酰胺柱层析 - 次分离,获得有效成分高良姜素.