suicide gene

The gene deletion technique include transposition bracketing method and the construction of suicide plasmid.

其中基因缺失技术还包括转座子插入法和自杀性质粒的构建。

Followed the prodrug fludarabine incubation, the cytotoxic effect, bystander effect and apoptosis induction of PNP/ fludarabine suicide gene system on BEL7402 cells were detected by methyl thiazolyl tetrazolium ( MTT) colorimetric method and Annexin V-FITC apoptosis detection kit, respectively.

分别应用四甲基偶氮唑蓝（MTT）比色法和Annexin V鄄FITC凋亡检测试剂盒检测PNP/fludarabine自杀基因系统对BEL7402细胞的细胞毒效应、旁观者效应和诱导凋亡作用。

Experimental Study on Colorectal Carcinoma Associate Double Suicide Gene Therapy with Radiotherapy

双自杀基因联合放疗对大肠癌治疗的实验研究

Suicide gene therapy, also known as gene-directed enzyme prodrug therapy, is an important strategy for tumor treatment by improving the specifical concentrations of drugs in tumors and a unique bystander effect.

自杀基因疗法，又名基因介导的酶-前药疗法，能特异性提高药物在肿瘤中的浓度，并具备独特的旁观者效应，是肿瘤基因治疗的重要方法之一。

Construction of HSV-tk/ GCV suicide gene system for hepatocarcinoma cells and its bystander effect in rats

大鼠肝癌细胞HSV-tk/GCV自杀基因系统的构建及其旁观者效应

Thereby, it was suggested that optimal gene therapeutic model might enhance the curative effect of suicide gene.

提示：优化胶质瘤基因治疗模式可进一步提高基因治疗效果。

The development of herpes simplex virus thymidine kinase suicide gene imaging

单纯疱疹病毒胸苷激酶自杀基因显像研究进展

Therefore enhancing the bystander effect has become an important strategy to raise curative effect of the suicide gene therapy.

因此，增强旁观者效应已成为提高肿瘤自杀基因治疗疗效的重要策略。

Bystander effect in suicide gene therapy for glioma

恶性胶质瘤自杀基因治疗中旁效应的机理

CD/ 5-FC suicide gene therapy system mediated by Bifidobacterium Infantis was noted to have good antitumor effect on melanoma.

婴儿双歧杆菌介导的CD/5-FC自杀基因系统对黑色素瘤具有明显的抑瘤效果。

Effects of CD/ 5-FC suicide gene therapy system on human malignant glioma cells in vitro

CD/5-FC自杀基因治疗系统体外杀伤恶性人脑胶质瘤细胞作用的研究

Relationship between the expression of connexin 43 and bystander effect of suicide gene therapy in ovarian cancer

卵巢癌自杀基因治疗的旁观者效应及其与间隙连接蛋白43表达的关系

Synergism of Liuwei Dihuang Bolus on Suicide Gene Therapy for Murine Transplanted Hepatocarcinoma

六味地黄丸对小鼠移植性肝癌自杀基因治疗的增效作用

Conclusion Recombinant Bifidobacterium Infantis with UPRT gene can significantly enhance the killing effect of CD/ 5-FC suicide gene system on melanoma B16-F10 cells of mice.

结论婴儿双歧杆菌联合转导UPRT基因可明显增强CD/5-FC自杀基因系统对鼠黑色素瘤细胞B16-F10的杀伤作用。

Killing effect of GCV combined with suicide gene HSV-tk on human osteosarcoma cells in vitro

HSV-tk/GCV自杀基因系统对骨肉瘤细胞体外杀伤效应

Killing Effect of Human Pulmonary Adenocarcinoma Cells with TK+ CD/ 5-Fc+ GCV Coexpression Suicide Gene Systems

自杀基因CD、TK的共表达对人肺腺癌细胞的杀伤作用

Experimental studies of using adenovirus-mediated cd/ 5-fc suicide gene in treatment of pan-creatic cancer

CD/5-FC自杀基因系统治疗胰腺癌的实验研究

Conclusion DAAO/ D Ala suicide gene system might be useful in cancer gene therapy.

结论DAAO D Ala自杀基因系统可以进一步用于肿瘤的基因治疗研究。

Investigate the Synergy of Liu Wei Di Huang Bolus on Hepatocarcinoma Suicide Gene Therapy from Gap Junction

从缝隙连接探讨六味地黄丸对自杀基因治疗肝癌的增效作用

The Enhancing Utility and Mechanism Research of Curcumin on Bystander Effect of HSV-tk/ GCV Suicide Gene System

姜黄素对tk/GCV自杀基因系统旁观者效应的增效作用及机制

Research and progress on suicide gene therapy for pancreatic tumor

自杀基因在胰腺癌治疗中的研究进展

Suicide gene therapy for glioma using HSV tk/ GCV system

HSV-tk/GCV系统对神经胶质瘤的自杀基因治疗研究

Enhancing Effect of Chinese Herbal Medicine Active Components on Suicide Gene Bystander Effect and the Mechanism Study

中药活性成分对自杀基因旁观者效应增效作用及机制研究

Experimental Study on Treatment of Colon Cancer with E. Coli. Cytosine Deaminase Suicide Gene

胞嘧啶脱氨酶自杀基因治疗大肠癌的实验研究

The suicide gene system has almost the bystander effect, but its mechanism has yet been unclear.

现已证明，几乎所有的自杀基因系统都具有旁观者效应，但其作用机制尚不十分清楚。

At present, the most mature research of suicide gene systems are HSV-TK/ GCV and CD/ 5-FC.

目前研究最为成熟的自杀基因系统是HSV-TK/GCV和CD/5-FC。

Objective: To explore therapeutic effect of a novel yeast cytosine deaminase ( YCD) suicide gene in vivo on P388/ DBA murine leukemia model.

目的：以P388/DBA小鼠白血病模型，探讨新型自杀基因YCD的体内治疗作用。

Conclusion HSV-TK/ GCV in combination with HCPT can enhance the lethal effect of suicide gene therapy against human bladder carcinoma cells and effectively induce apoptosis of the cells.

结论HSV-TK/GCV系统联合HCPT治疗能增强自杀基因系统对人膀胱癌的杀伤作用，能够良好地诱导人膀胱癌细胞的凋亡。