whole genome sequencing

the whole genome dna sequencing is basic to the study of bioinformatics and in the commonly used shotgun method the reconstruction of the original sequence by the information of all the fragments is one of the important steps.

全基因组DNA序列测定是生物信息学研究的基础，在普遍使用的Shotgun方法中，其中一个重要的步骤就是根据片段信息来重构原始序列。

arabidopsis has good characteristics of small genome, simple structure, small plant, short life period, high propagating rate, self-pollinating etc. and the whole genome sequencing has been completed in december 2000. so it is the model plant for studying plant and pathogen interaction.

拟南芥具有基因组小、结构简单、形体小、生长周期短、繁殖系数高、自花授粉等优点，且其基因组全序列测定工作已于2000年12月完成。

while in china, researches about cyanophage diversity were focused on cyanophage isolations, whole genome sequencing and so on in the freshwater, no information about the genetic diversity of marine cyanophages has been reported.

在我国噬藻体的研究主要集中在淡水噬藻体的分离、全基因组的测序等方面，未有对海洋噬藻体多样性方面的研究报道。

two strategies introduced for whole genome sequencing, one is clone by clone method, the other is whole genome shotgun sequencing, for microbes which are very important to us, whole genome shotgun sequencing method is very convenient.

全基因组测序主要有二种策略，一种是分级鸟枪法测序，另一种是全基因组鸟枪法测序。

whole microbial genome shotgun sequencing

微生物全基因组鸟枪法测序

Whole genome Sequencing and the Comparative Genomics of Free Living Single Cell Organisms

单细胞自由生物基因组全序列的测定和比较基因组研究

the accomplishment of the whole rice genome sequencing project was a very important milestone in the history of rice gene and genomics studies.

水稻全基因组测序工作的完成是水稻基因和基因组研究史上的一个重要里程碑。

the use of full-length cdna libraries is an effective tool to obtain complete gene information in a high-efficiency, high-throughput manner, especially in organisms with huge genomes that are not amenable to whole genome sequencing.

全长cDNA文库是高效、大规模获得基因全序列信息的一条有效途径，尤其是对基因组庞大、近期内尚不能进行全基因组测序的生物来说，是一条开展功能基因组研究的重要途径。

with the development of the next-generation sequencing technology, whole genome and exome sequencing developed rapidly and provide an opportunity for us to deal with the problem caused by gwas.

而新一代测序技术的进步，促进了全基因组测序和全基因组外显子测序的快速发展，为解决上述问题提供了契机。

recent developments in analytical methods for protein characterization, and the growing rate at which whole genome sequencing projects are being completed, have combined to support the emergence and development of the new field of 'proteomics'.

蛋白质鉴定技术的发展与基因组测序计划的实施与相继完成，使得蛋白质组学这一全新的研究领域得以诞生和发展。

as one of the most important alimentary crops, rice has a small genome and is easy to do gene transform. in addition, its whole genome sequencing was completed. therefore, as a model monocot, rice is an ideal experimental material.

另一方面，作为最重要的粮食作物之一，水稻具有基因组小，易于进行转基因操作等优点，并且已经完成了全基因测序，因此，水稻作为单子叶模式植物，为我们提供了理想的实验材料。

because of its large genome and polyploidy, it is difficult to complete the whole genome sequencing and sequence assembly.

但由于其基因组较大、多倍性等原因，全基因组的测序及拼接工作难度较大。