ICSI

Objective: To determine an optimal insemination technique for unexplained infertility patients undergoing IVF or ICSI following failed intrauterine insemination ( IUI).

目的：探讨对不明原因原发性不孕患者行宫腔内人工授精（IUI）失败后治疗方法的合理选择。

Methods: To treat the male infertility by ICSI ( introcytoplasmic sperm injection), at the same time carry through the series basic reseach: the hatching efficacy of human embryos subjected to laser zona pellucida thinning;

方法：采用显微受精试管婴儿（卵胞浆内单精子显微注射，ICSI）治疗男性不孕症，同时进行了系列基础研究：显微激光薄化人胚胎透明带进行辅助孵出的效果研究；

ICSI is so remarkable, in fact, that most treatments previously used have been abandoned in its favor.

卵胞浆内单精子注射是如此卓越，事实上，由于对这种方法的认可，大多数以前的治疗方法已经被取替。

Objective: Retrospective study of the results of ICSI ( intracytoplasmic sperm insemination) with frozen sperm obtained by PESA ( percutaneous epididymal sperm aspiration) was performed in 27 patients.

目的：回顾性分析27例无精子症患者经皮附睾穿刺取精术（PESA）所获精子冷冻复苏后行卵细胞胞质内单精子注射（ICSI）治疗后的效果及妊娠结局。

to study the impact of sperm originations on the therapeutic outcome of icsi.

研究不同来源的精子对于ICSI辅助生殖助孕结局的影响。

In order to attempt pregnancy, egg freezing must be followed by in vitro fertilization ( IVF) with another laboratory procedure, a technique known as ICSI ( Intracytoplasmic Sperm Injection).

为了怀孕，卵子冷冻必须配合另外一套实验室程序&体外受精（IVF），该项技术被称为ICSI（卵母细胞胞浆内单精子注射）。

In1996, our country's first ICSI test-tube baby in sun yat-sen university, the first hospital affiliated reproductive medicine center was born.

1996年我国首例ICSI试管婴儿在中山大学附属第一医院生殖医学中心诞生。

Patients undertaken IVF-ET or ICSI in Group C ( 35 cycles) had oocyte-retrieved the same time as Group A.

C组（35周期）选择与A组同期取卵进行体外受精-胚胎移植（IVF）或卵细胞浆单精子注射（ICSI）者。

Objective: To describe sperm preparation methods of ejaculated, epididymal and testicular spermatozoa in intracytoplasmic sperm injection ( ICSI).

目的：介绍单精子卵胞浆内注射术（ICSI）中不同来源精子的洗涤处理方法。

Conclusion TESE with ICSI is an effective way to treat irreparable obstructive azoospermic infertility.

结论tese结合ICSI是治疗男性梗阻性无精子所致不育症的有效方法.

10% bff could significantly increase the blastocyst rate and bff was more efficient than fbs for the early development in vitro of goat icsi embryos.

在早期胚胎培养中，添加10%BFF可显著提高ICSI胚的囊胚发育率；添加10%BFF比添加10%FBS对提高早期胚胎培养质量更为有效。

Conclusion The ejaculated, epididymal aspirates and testicular sperm used in ICSI achieved comparable clinical results.

结论不同来源精子行ICSI治疗男性因素不育可以获得相似的治疗结果。

Conclusions ICSI with PESA or TESE is an effective method for treatment of obstructive azoospermic patients.

结论采用附睾/睾丸精子行ICSI是治疗男性阻塞性无精子症的有效方法。

methods: sibling oocyte cumulus complexes ( occ) of 43 different couples were divided into two groups: ivf group and icsi group, using the same semen sample and inseminated respectively by ivf and by icsi ( ivf+ icsi).

方法：选择我中心实施体外受精胚胎移植中的43个治疗周期，将同周期同胞卵分成IVF组和ICSI组，使用相同的精液标本分别实施IVF和ICSI（IVF+ICSI），进行比较分析。

Objective: To evaluate the efficiency of density gradient and improved swim-up methods for motile sperm isolation from fresh semen samples in intracytoplasmic sperm injection and embryo transfer ( ICSI-ET) program, thus guiding the clinical application.

目的：评价密度梯度离心和改良上游法两种处理活动精子的分离方法在卵细胞胞质内单精子显微注射（ICSI）中的效果，从而指导临床应用。

Methods : The data of 79 cases of ICSI and 288 cases of IVF were analysed retrospectively.

方法回顾性分析79例接受技术治疗不育的资料,并与同期288例接受常规的体外受精与胚胎移植(IVF-ET)技术的病例对比分析.

Follicular development potential which could be the difference in maturation rate of IVM group, the rate of excellent embryos, the pregnancy rate than IVF/ ICSI group an important reason.

其中卵泡发育潜能、内分泌紊乱的后遗效应、内膜成熟的差异可能是导致IVM组成熟率、优胚率、妊娠率低于IVF/ICSI组的重要原因。

Conclusion: Time phase of controlled ovarian hyperstimulation is correlated with clinical pregnancy rate of IVF-ET or ICSI. Proper duration of gonadotropin stimulation can influence embryo quality, appropriate hCG day can synchronize embryo growth and endometrium.

结论IVF-ET及ICSI的临床妊娠率与促超排卵的时相相关，适当的Gn使用天数可以提高胚胎质量、适宜的hCG日则通过同步胚胎与子宫内膜的发育发挥作用。

Dead spermatozoa were capable of fertilizing oocytes by ICSI, but rates of fertilization and embryo development declined with time after death.

山羊死亡精子通过ICSI技术可以受精并发育，但是随死亡时间延长受精和发育能力下降。

Conclusion ICSI is an effective treatment for infertility of severe oligo-astheno-teratospermia and obstructive azoospermia.

结论ICSI技术是治疗重度少、弱、畸精症及阻塞性无精症性不育的有效手段。

Treatment choice of ICSI was not associated with fertilization, pregnancy, or live birth rates.

选择ICSI治疗与受精 、 妊娠及出生率并无相关性.

Methods: ICSI and SUZI were employed to obtain the assisted fertilization of mouse sperm from caput epididymidis and cauda epididymidis.

方法：用显微注射法把小鼠附睾头和附睾尾精子注入卵母细胞的胞质内或卵周隙进行显微受精。

When freezing single testicular spermatozoa, cryoprotectant-free vitrification was safety, economical and effective, and the fertility rate was similar to fresh sperm in ICSI.

采用不加冷冻保护剂的玻璃化法冷冻保存微量睾丸精子是一种安全、经济、有效的冷冻保存方法，冷冻复苏后采用ICSI技术可取得与新鲜精子相似的受精率。

Effect of Different Single Sperm Injection Method for ICSI on in vitro Development of Embryos

ICSI时不同单精子注入方式对胚胎体外发育的影响

We compared patient characteristics, cycle details, and outcomes for ICSI and IVF.

比较了病人的特征, 各个周期的特点及ICSI和IVF的结局.