calli

Calli containing very low IAA or ABA would become brown and die in their subculturing.

IAA和ABA水平过低的愈伤组织继代时会逐渐褐化、死亡；

Calli were induced and suspension cell lines were set up from ovule of Malus pumila Mill.

用苹果（Malus pumila Mill）胚珠诱导愈伤组织并建立悬浮细胞系。

Response of Protective Enzymes in Calli of Barnyard Grass to Oxidizing Stress Induced by α-terthienyl

光活化α-三噻吩诱发稗草愈伤组织保护酶系的反应

CONCLUSION: The callus growth was stimulated by the low concentrations of rare earth elements. The lower and higher concentrations of Yb 3+ and Eu 3+ had negative effect on the continuing growth of the calli transferred on the medium free of rare earth elements.

结论：低浓度的稀土化合物对黄连愈伤组织的生长具有促进作用，低浓度和高浓度的Yb3＋和Eu3＋对黄连愈伤组织后续的继代培养均有不利的影响。

Using embryogenic calli from young embryos as explants, bean chitinase gene was transferred into spring wheat plants by microprojectile bombardment.

利用基因枪法，以菜豆几丁质酶基因转化小麦幼胚愈伤组织。

The establishment of blackwood calli system.

4.黑木相思愈伤组织体系的建立.

Factors Influencing Induction and Subculture of Calli from Maize Mature Embryo

玉米成熟胚愈伤组织诱导与继代培养的影响因素研究

The histochemical analysis of gus gene activity indicated that Calli of transient expression at 53.08% to show blue, the Chi gene and reporter gene gus are in the same open reading frame, accordingly we deduced that chi gene could express also.

gus基因表达的组织化学染色表明，gus基因瞬时表达率最高可达到53.08%的愈伤组织呈现蓝色，Chi基因与报告基因gus处于同一开放阅读框内，由此推断Chi基因也能表达。

Using Aegilops crassa cytoplasm wheat as test material, salt tolerance of alloplasmic wheat was identified by tissue culture, nutrient solution culture and artificial saline pond, from calli, seeding stage, recovering stage and mature period.

以粗厚山羊草（Aegilops crassa）细胞质小麦为材料，采用组织培养、水培、模拟盐池等方法，研究细胞质对小麦耐盐性的遗传效应。

The calling convention is not checked dynamically, so code that uses a calli instruction does not work correctly if the destination does not actually use the specified calling convention.

不动态检查调用约定，因此如果目标实际上没有使用指定的调用约定，则使用calli指令的代码将不会正确工作。

The habituated nucellar calli consisted of embryogenic and non-embryogenic cells.

电镜和光镜观察表明，适应型珠心愈伤组织由胚性细胞和非胚性细胞组成。

Salt tolerance assays demonstrated that the salt tolerance of transgenic calli and regenerated plantlets were increased.

经DNA杂交 证实的转基因植株被用于耐盐性试验,结果表明这些转基因的植株的耐盐性有明显的提高.

Methods of Lowering Temperature for Cryopreservation of Calli of Robinia bella-rosea

二乔刺槐愈伤组织超低温保存及适宜降温方法

The callus increment of fresh weight was lower than the control when the calli, derived from the media containing various concentrations of Yb 3+ and Eu 3+, were transferred and cultured on the medium free of rare earth elements.

在不含稀土化合物的培养基上的继代培养中，来源于稀土化合物处理的愈伤组织鲜重增加值均比对照低。

Cell cycle analysis can provide reference for researching the physical and chemical mutagenesis to citrus calli.

细胞周期分析可为研究柑橘愈伤组织理化诱变提供依据.

The plant expression vector carrying the herbicide resistance bar gene was introduced into wheat immature embryo calli using particle bombardment. Parameters of bombardment were optimized by GUS transient expression, such as precipitants, distance, and amount of gold powder.

用基因枪法将含有抗除草剂bar基因的植物表达载体导入优良品种小麦幼胚愈伤组织，并通过GUS瞬时表达对沉淀剂、轰击距离、金粉用量等轰击参数进行了优化。

While the non-embryogenic calli consisted of non-regular shaped cells with big central vacuole and thin cytoplasm.

而非胚性愈伤组织由无规则状态的细胞组成，高度液泡化，胞质稀少。

Rice chitinase gene was transferred into immature embryogenic calli of wheat using PDS1000/ He particle delivery system.

利用PDS1000/氦气基因枪将水稻几丁质酶基因导入小麦幼胚盾片愈伤组织。

This article studied the main factors of influencing the induction of rice scutella and calli.

本文以水稻成熟种子为材料，研究了影响其盾片诱导、愈伤组织诱导及其分化的主要因素，建立了籼稻愈伤组织及其再生体系。

For γ-ray treatment of wheat immature embryo calli, a dosage of 1000-1200 rads was con-sidered suitable.

以~（60）Co&γ射线辐照小麦幼胚愈伤组织，辐照剂量以1000～1200拉德为宜。

However, embryogenic capability may be gradually lost during the preservation of calli.

但植物愈伤组织在保存过程中会逐渐丧失胚性能力。

Adding of BAP in callus induction medium shortened the time of shoot emergence but could not improve the producing of embryogenic calli and green plantlets.

愈伤诱导培养基中添加一定浓度的BAP不能提高胚性愈伤率和再生频率，但能够加速芽苗的出现。

The meristem of type B differentiated later and had more meristem tissue than type A. We have observed mature somatic embryogenesis in the materials incubated on the medium II. The white calli changed to green.

在培养基Ⅱ中，观察到了成熟的体细胞胚，40d后白色的胚性愈伤组织转绿。

The calli produced buds on differentiation medium.

愈伤组织在分化培养基上产生幼芽.

Hormone The green shoot-bud regeneration rates of calli were affected by combinations of different auxins in regeneration medium.

分化培养基上不同激素配比也影响其绿苗再生率。

The stem explants of sweet potato were used to induce calli.

在本论文的研究工作中，将甘薯的茎段在特定条件下，诱导培养甘薯愈伤组织。

The plant expression vector carrying a herbicide resistance BAR gene and a marker GUS gene was transferred into wheat spikelets and immature embryo calli via Agrobacterium tumefaciens Ti plasmid and microprojectile bombardment.

作者通过根癌农杆菌（Agrobacterium tumefaciens）Ti质粒介导和基因枪法，将含抗除草剂Bar基因的植物表达载体，导入小麦幼穗和幼胚愈伤组织。

Study on the Recidivism System of Units Calli Induction of Polygonum multiflorum Thunb

论单位累犯何首乌愈伤组织的诱导

To test whether DNA methylation plays a role in somaclonal variation, the calli were treated with 5-azacytidine that reduces cytosine methylation by blocking activity of DNA methyltransferase.

为了检测DNA甲基化在体细胞克隆多样性中的作用，愈伤组织用5-氮胞苷进行处理，5-氮胞苷通过抑制DNA甲基转移酶的活性降低胞嘧啶的甲基化水平。

Results Two mannitol-tolerant cell lines were obtained from the embryogenic calli using positive selection method.

以所得到的胚性愈伤组织为起始材料，采用一步和多步正筛选法离体筛选耐甘露醇变异细胞系。