rT的例句大全

Conclusions The human leptin gene cDNA clone was constructed by RT PCR.

【结论】以逆转录聚合酶链反应方法成功地构建了人瘦素基因cDNA克隆。

The rats of group B were treated with EPO after globe ischemia-reperfusion. Brain tissues were taken out after execution at different time point. The expression of caspase-9 mRNA was detected with reverse transcription PCR ( RT-PCR) technique.

在相应时间点断头取脑，逆转录聚合酶链反应技术检测大鼠皮质caspase9mRNA的表达，并与对照组（C组）比较。

Rapid extraction of enterovirus RNA for RT-PCR

快速提取肠道病毒RNA用于逆转录聚合酶链反应

Methods R and/ or G banding technique was used for analysis of karyotype. DNA blotting for HRX gene rearrangement, and polymerase chain reaction ( PCR) and reverse transcriptase PCR ( RT PCR) for fusion gene detection.

方法用R带和（或）G带分带技术进行核型分析，DNA印迹法检测HRX重排，聚合酶链反应和逆转录聚合酶链反应进行融合基因检测。

Methods: The mRNA was extracted from the fresh tissues of 16 colonic cancer patients; reverse transcriptase polymerase chain reaction ( RT-PCR) was used to detect the receptors of TRAIL.

方法：取16例新鲜结肠癌组织提取mRNA，用逆转录聚合酶链反应（RT-PCR）方法检测TRAIL受体的表达。

The expression of epidermal growth factor receptor was detected in the keratinocytes with immunocytochemistry and the transcription of epidermal growth factor receptor mRNA was determined with reverse transcription-polymerase chain reaction ( RT-PCR).

采用免疫细胞化学的方法检测表皮生长因子受体的表达，采用复合逆转录聚合酶链反应检测角质形成细胞中表皮生长因子受体mRNA的表达。

The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction ( RT-PCR) and Western blot.

采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。

METHODS: A sensitive nested-RT-PCR system was set up to make relative quantitative values of AFP mRNA of hepatic tissues and peripheral blood.

方法：建立敏感的巢式逆转录聚合酶链反应体系（Nested RT PCR），对肝组织和外周血中的AFP mRNA进行相对定量值分析。

The RNA of islets in three purity was extracted, amplified with RT-PCR, and the mRNA expression of stem cell markers CK-19 and PDX-1 was detected.

分别将上述3组纯化后的胰岛抽提RNA行逆转录聚合酶链反应（RT-PCR），分析胰岛干细胞标志物CK-19及PDX-1mRNA的表达。

The standard testing technique for Ebola is known as reverse transcription polymerase chain reaction, or RT-PCR.

标准的埃博拉检测技术被称作逆转录聚合酶链反应，简称RT-PCR。

Serum myocardial enzymes was determined by biochemical method and the expression of c-fos mRNA in myocardium was detected by using reverse transcription polymerase chain reaction ( RT-PCR).

用生化方法检测血清心肌酶活性，逆转录聚合酶链反应（RT-PCR）方法检测心肌中c-fos基因的表达。

And semi-quantification reverse transcriptase-polymerase chase reaction ( RT-PCR) was used to analyse the effects of CIM and BET on mRNA expression of histamine receptors in the tissue of hepatocarcinoma.

并以逆转录聚合酶链反应（RT-PCR）半定量法研究BET、CIM对肝癌中组胺受体mRNA表达的影响。

To integrate the results of clustering data mining and based on visual model requirement, network structure diagram of HQDS would be drawn, gene network structure be study with RT-PCR.

结合挖掘结果，运用网络结构可视化思想构建心气虚证网络结构图，并运用逆转录聚合酶链反应（RT-PCR）探索心气虚证基因网络结构。

METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.

我们用A549细胞研究PARs在肺上皮细胞的表达.方法：采用逆转录聚合酶链反应（RT-PCR）、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系A549细胞PARs的表达情况。

Immunocytochemistry showed that there was an evident expression of epidermal growth factor receptor in the keratinocytes. The transcription of epidermal growth factor receptor mRNA was apparent according to RT-PCR.

免疫细胞化学显示表皮生长因子受体在细胞表面有明显的表达，复合逆转录聚合酶链反应显示表皮生长因子受体mRNA有明显的表达。

Using reverse transcription polymerase chain reaction ( RT-PCR), CCK-A receptor ( CCK-AR) and CCK-B receptor ( CCK-BR) mRNA were expressed in myocardium of rats.

由逆转录聚合酶链反应（RT PCR）检测到心肌组织有CCK A受体（CCK AR）和CCK B受体（CCK BR）mRNA表达。

Objective To establish the real-time RT-PCR method to detect the mRNA level of dihydropyrimidine dehydrogenase, and to determine the expression of dihydropyrimidine dehydrogenase in various human tissues in RNA level and protein level.

目的建立实时逆转录聚合酶链反应（RT PCR）检测二氢嘧啶脱氢酶mRNA的定量方法，并分别从RNA水平与蛋白质水平检测二氢嘧啶脱氢酶在人体部分组织中的表达。

Methods: The blood samples were collected from HFRS patients between 2000~ 2001, and the M segment genome of hantavirus was amplified by RT-PCR.

方法：采集2000~2001年肾综合征出血热（HFRS）患者的血液标本，通过逆转录聚合酶链反应（RT-PCR）扩增汉坦病毒M片断基因。

The α 3 and α 7 nAChR subunits in protein level were measured by Western blotting and in mRNA level by RT-polymerase chain reaction ( RT-PCR).

用蛋白印记方法测定α3和α7尼古丁受体亚单位蛋白水平；用逆转录聚合酶链反应方法测定尼古丁受体亚单位mRNA水平。

Reverse transcription-polymerase chain reaction ( RT-PCR) was developed for the detection of dengue virus 3 genome.

本文应用逆转录聚合酶链反应（RT-PCR）方法，检测Ⅲ型登革病毒基因。